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Structural basis of outer membrane protein insertion by the BAM complex

机译：BAM复合物插入外膜蛋白的结构基础

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摘要

All Gram-negative bacteria, mitochondria and chloroplasts have outer membrane proteins (OMPs) that perform many fundamental biological processes. The OMPs in Gram-negative bacteria are inserted and folded into the outer membrane by the beta-barrel assembly machinery (BAM). The mechanism involved is poorly understood, owing to the absence of a structure of the entire BAM complex. Here we report two crystal structures of the Escherichia coli BAM complex in two distinct states: an inward-open state and a lateral-open state. Our structures reveal that the five polypeptide transport-associated domains of BamA form a ring architecture with four associated lipoproteins, BamB-BamE, in the periplasm. Our structural, functional studies and molecular dynamics simulations indicate that these subunits rotate with respect to the integral membrane beta-barrel of BamA to induce movement of the beta-strands of the barrel and promote insertion of the nascent OMP.
机译：所有革兰氏阴性细菌，线粒体和叶绿体均具有执行许多基本生物学过程的外膜蛋白（OMP）。革兰氏阴性细菌中的OMP通过β-桶装配机器（BAM）插入并折叠到外膜中。由于缺乏整个BAM复合体的结构，因此对所涉及的机制了解甚少。在这里，我们报告了两种不同状态的大肠杆菌BAM复合物的两个晶体结构：向内打开状态和侧向打开状态。我们的结构揭示了BamA的五个与多肽运输相关的结构域与周质中的四个相关脂蛋白BamB-BamE形成了环结构。我们的结构，功能研究和分子动力学模拟表明，这些亚基相对于BamA的整体膜β-桶旋转，以诱导桶中β-链的运动并促进新生OMP的插入。

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  • 来源
    《Nature》 |2016年第7592期|64-69|共6页
  • 作者

    Gu Yinghong; Li Huanyu; Dong Haohao; Zeng Yi; Zhang Zhengyu; Paterson Neil G.; Stansfeld Phillip J.; Wang Zhongshan; Zhang Yizheng; Wang Wenjian; Dong Changjiang;

  • 作者单位

    Univ E Anglia, Norwich Med Sch, Biomed Res Ctr, Norwich Res Pk, Norwich NR4 7TJ, Norfolk, England;

    Univ E Anglia, Norwich Med Sch, Biomed Res Ctr, Norwich Res Pk, Norwich NR4 7TJ, Norfolk, England;

    Univ E Anglia, Norwich Med Sch, Biomed Res Ctr, Norwich Res Pk, Norwich NR4 7TJ, Norfolk, England;

    Univ E Anglia, Norwich Med Sch, Biomed Res Ctr, Norwich Res Pk, Norwich NR4 7TJ, Norfolk, England;

    Univ E Anglia, Norwich Med Sch, Biomed Res Ctr, Norwich Res Pk, Norwich NR4 7TJ, Norfolk, England;

    Diamond Light Source, Harwell Sci & Innovat Campus, Didcot OX11 0DE, Oxon, England;

    Univ Oxford, Dept Biochem, S Parks Rd, Oxford OX1 3QU, England;

    Univ E Anglia, Norwich Med Sch, Biomed Res Ctr, Norwich Res Pk, Norwich NR4 7TJ, Norfolk, England|Xuzhou Med Coll, Jiangsu Prov Key Lab Anesthesiol, Xuzhou 221004, Peoples R China|Sichuan Univ, Coll Life Sci, Key Lab Bioresources & Ecoenvironm, Minist Educ,Sichuan Key Lab Mol Biol & Biotechnol, Chengdu 610064, Peoples R China;

    Sichuan Univ, Coll Life Sci, Key Lab Bioresources & Ecoenvironm, Minist Educ,Sichuan Key Lab Mol Biol & Biotechnol, Chengdu 610064, Peoples R China;

    Sun Yat Sen Univ, Affiliated Hosp 1, Lab Dept Surg, 58 Zhongshan Rd 2, Guangzhou 510080, Guangdong, Peoples R China;

    Univ E Anglia, Norwich Med Sch, Biomed Res Ctr, Norwich Res Pk, Norwich NR4 7TJ, Norfolk, England;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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