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Priming and polymerization of a bacterial contractile tail structure

机译：细菌收缩尾结构的引发和聚合

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摘要

Contractile tails are composed of an inner tube wrapped by an outer sheath assembled in an extended, metastable conformation that stores mechanical energy necessary for its contraction. Contraction is used to propel the rigid inner tube towards target cells for DNA or toxin delivery. Although recent studies have revealed the structure of the contractile sheath of the type VI secretion system, the mechanisms by which its polymerization is controlled and coordinated with the assembly of the inner tube remain unknown. Here we show that the starfish-like TssA dodecameric complex interacts with tube and sheath components. Fluorescence microscopy experiments in enteroaggregative Escherichia coli reveal that TssA binds first to the type VI secretion system membrane core complex and then initiates tail polymerization. TssA remains at the tip of the growing structure and incorporates new tube and sheath blocks. On the basis of these results, we propose that TssA primes and coordinates tail tube and sheath biogenesis.
机译：收缩的尾巴由包裹有外套管的内管组成，外套管以扩展的亚稳态构型组装，该构型存​​储了其收缩所需的机械能。收缩用于将刚性内管推向目标细胞，以供DNA或毒素输送。尽管最近的研究已经揭示了VI型分泌系统的收缩鞘的结构，但其聚合受到控制并与内管组装相协调的机理仍然未知。在这里，我们显示了类似海星的TssA十二聚体复合物与管和鞘成分相互作用。在肠聚合性大肠杆菌中的荧光显微镜实验表明，TssA首先与VI型分泌系统膜核心复合物结合，然后引发尾部聚合。 TssA仍处于增长结构的尖端，并结合了新的管和护套块。基于这些结果，我们建议TssA启动并协调尾管和鞘的生物发生。

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  • 来源
    《Nature》 |2016年第7592期|59-63|共5页
  • 作者

    Zoued Abdelrahim; Durand Eric; Brunet Yannick R.; Spinelli Silvia; Douzi Badreddine; Guzzo Mathilde; Flaugnatti Nicolas; Legrand Pierre; Journet Laure; Fronzes Remi; Mignot Tam; Cambillau Christian; Cascales Eric;

  • 作者单位

    Aix Marseille Univ, CNRS, UMR7255, Lab Ingn Syst Macromol,Inst Microbiol Mediterrane, 31 Chemin Joseph Aiguier, F-13402 Marseille 20, France;

    Aix Marseille Univ, CNRS, UMR7255, Lab Ingn Syst Macromol,Inst Microbiol Mediterrane, 31 Chemin Joseph Aiguier, F-13402 Marseille 20, France|CNRS, UMR 7257, Architecture & Fonct Macromol Biol, Campus Luminy,Case 932, F-13288 Marseille 09, France|Aix Marseille Univ, UMR 7257, Architecture & Fonct Macromol Biol, Campus Luminy,Case 932, F-13288 Marseille 09, France|Inst Pasteur, Biol Struct Secret Bacterienne G5, 25-28 Rue Docteur Roux, F-75015 Paris, France|Inst Pasteur, CNRS, UMR 3528, 25-28 Rue Docteur Roux, F-75015 Paris, France;

    Aix Marseille Univ, CNRS, UMR7255, Lab Ingn Syst Macromol,Inst Microbiol Mediterrane, 31 Chemin Joseph Aiguier, F-13402 Marseille 20, France|Harvard Univ, Sch Med, Dept Microbiol & Immunobiol, 77 Ave Louis Pasteur, Boston, MA 02115 USA;

    CNRS, UMR 7257, Architecture & Fonct Macromol Biol, Campus Luminy,Case 932, F-13288 Marseille 09, France|Aix Marseille Univ, UMR 7257, Architecture & Fonct Macromol Biol, Campus Luminy,Case 932, F-13288 Marseille 09, France;

    Aix Marseille Univ, CNRS, UMR7255, Lab Ingn Syst Macromol,Inst Microbiol Mediterrane, 31 Chemin Joseph Aiguier, F-13402 Marseille 20, France|CNRS, UMR 7257, Architecture & Fonct Macromol Biol, Campus Luminy,Case 932, F-13288 Marseille 09, France|Aix Marseille Univ, UMR 7257, Architecture & Fonct Macromol Biol, Campus Luminy,Case 932, F-13288 Marseille 09, France;

    Aix Marseille Univ, CNRS, UMR7283, Lab Chim Bacterienne,Inst Microbiol Mediterranee, 31 Chemin Joseph Aiguier, F-13402 Marseille 20, France;

    Aix Marseille Univ, CNRS, UMR7255, Lab Ingn Syst Macromol,Inst Microbiol Mediterrane, 31 Chemin Joseph Aiguier, F-13402 Marseille 20, France;

    Synchrotron Soleil, BP48, F-91192 Gif Sur Yvette, France;

    Aix Marseille Univ, CNRS, UMR7255, Lab Ingn Syst Macromol,Inst Microbiol Mediterrane, 31 Chemin Joseph Aiguier, F-13402 Marseille 20, France;

    Inst Pasteur, Biol Struct Secret Bacterienne G5, 25-28 Rue Docteur Roux, F-75015 Paris, France|Inst Pasteur, CNRS, UMR 3528, 25-28 Rue Docteur Roux, F-75015 Paris, France;

    Aix Marseille Univ, CNRS, UMR7283, Lab Chim Bacterienne,Inst Microbiol Mediterranee, 31 Chemin Joseph Aiguier, F-13402 Marseille 20, France;

    CNRS, UMR 7257, Architecture & Fonct Macromol Biol, Campus Luminy,Case 932, F-13288 Marseille 09, France|Aix Marseille Univ, UMR 7257, Architecture & Fonct Macromol Biol, Campus Luminy,Case 932, F-13288 Marseille 09, France;

    Aix Marseille Univ, CNRS, UMR7255, Lab Ingn Syst Macromol,Inst Microbiol Mediterrane, 31 Chemin Joseph Aiguier, F-13402 Marseille 20, France;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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