• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nature >MIMIVIRE is a defence system in mimivirus that confers resistance to virophage
【24h】

MIMIVIRE is a defence system in mimivirus that confers resistance to virophage

机译：MIMIVIRE是模仿病毒的防御系统，可赋予对噬菌体的抗性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Since their discovery, giant viruses have revealed several unique features that challenge the conventional definition of a virus, such as their large and complex genomes, their infection by virophages and their presence of transferable short element transpovirons(1-5). Here we investigate the sensitivity of mimivirus to virophage infection in a collection of 59 viral strains and demonstrate lineage specificity in the resistance of mimivirus to Zamilon(6), a unique virophage that can infect lineages B and C of mimivirus but not lineage A. We hypothesized that mimiviruses harbour a defence mechanism resembling the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas system that is widely present in bacteria and archaea(7-10). We performed de novo sequencing of 45 new mimivirus strains and searched for sequences specific to Zamilon in a total of 60 mimivirus genomes. We found that lineage A strains are resistant to Zamilon and contain the insertion of a repeated Zamilon sequence within an operon, here named the 'mimivirus virophage resistance element' (MIMIVIRE). Further analyses of the surrounding sequences showed that this locus is reminiscent of a defence mechanism related to the CRISPR-Cas system. Silencing the repeated sequence and the MIMIVIRE genes restores mimivirus susceptibility to Zamilon. The MIMIVIRE proteins possess the typical functions (nuclease and helicase) involved in the degradation of foreign nucleic acids. The viral defence system, MIMIVIRE, represents a nucleic-acid-based immunity against virophage infection.
机译：自发现以来，巨型病毒已经揭示了一些挑战病毒常规定义的独特特征，例如它们庞大而复杂的基因组，它们被病毒噬菌体感染以及存在可转移的短元件转座子病毒（1-5）。在这里，我们研究了59株病毒株中拟南芥病毒对噬菌体感染的敏感性，并证明了拟南芥对Zamilon（6）的抗性具有独特的谱系特异性，可以感染拟南芥B和C的拟南芥病毒，但不会感染A.假设mimiviruses具有类似于簇状规则间隔的短回文重复（CRISPR）-Cas系统的防御机制，该系统广泛存在于细菌和古细菌中（7-10）。我们对45个新的mivirvirus株进行了从头测序，并在总共60个mimivirus基因组中搜索了针对Zamilon的序列。我们发现，谱系A株对Zamilon具有抗性，并在操纵子内插入重复的Zamilon序列，此处称为“拟病毒抗病毒体元件”（MIMIVIRE）。对周围序列的进一步分析表明，该基因座使人联想到与CRISPR-Cas系统有关的防御机制。沉默重复的序列和MIMIVIRE基因可恢复拟病毒对Zamilon的敏感性。 MIMIVIRE蛋白具有参与外源核酸降解的典型功能（核酸酶和解旋酶）。病毒防御系统MIMIVIRE代表了针对病毒感染的基于核酸的免疫力。

著录项

  • 来源
    《Nature》 |2016年第7593期|249-252|共4页
  • 作者

    Levasseur Anthony; Bekliz Meriem; Chabriere Eric; Pontarotti Pierre; La Scola Bernard; Raoult Didier;

  • 作者单位

    Aix Marseille Univ, URMITE, INSERM, UM63,CNRS 7278,IRD 198,U1095, Marseille, France|IHU Mediterranee Infect, Assistance Publ Hop Marseille, Fac Med, 27 Blvd Jean Moulin, F-13005 Marseille, France;

    Aix Marseille Univ, URMITE, INSERM, UM63,CNRS 7278,IRD 198,U1095, Marseille, France|IHU Mediterranee Infect, Assistance Publ Hop Marseille, Fac Med, 27 Blvd Jean Moulin, F-13005 Marseille, France;

    Aix Marseille Univ, URMITE, INSERM, UM63,CNRS 7278,IRD 198,U1095, Marseille, France|IHU Mediterranee Infect, Assistance Publ Hop Marseille, Fac Med, 27 Blvd Jean Moulin, F-13005 Marseille, France;

    Aix Marseille Univ, CNRS, Cent Marseille, I2M,UMR7373,FR 4213,FR Eccorev 3098,Equipe EBM, F-13331 Marseille, France;

    Aix Marseille Univ, URMITE, INSERM, UM63,CNRS 7278,IRD 198,U1095, Marseille, France|IHU Mediterranee Infect, Assistance Publ Hop Marseille, Fac Med, 27 Blvd Jean Moulin, F-13005 Marseille, France;

    Aix Marseille Univ, URMITE, INSERM, UM63,CNRS 7278,IRD 198,U1095, Marseille, France|IHU Mediterranee Infect, Assistance Publ Hop Marseille, Fac Med, 27 Blvd Jean Moulin, F-13005 Marseille, France;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号