• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nature >Biocontainment of genetically modified organisms by synthetic protein design
【24h】

Biocontainment of genetically modified organisms by synthetic protein design

机译：通过合成蛋白设计对转基因生物进行生物控制

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

本期Nature上发表的两篇论文描述了生成一种依赖于非天然氨基酸的生物的独立方法，这一成果在生物封存(biocontainment)和探索以前未曾尝试过的健身方法方面可能会找到应用。George Church及同事通过一个被改变的基因代码对一种生物（大肠杆菌）的必要酶进行了重新设计，以使其在代谢上依赖于非标准氨基酸来存活。这样得到的转基因生物在代谢上无法绕开它们的生物封存机制，并且对演化逃避(evolutionary escape)也表现出前所未有的抵抗力。少数逃避者(escapees)很快被未转基因的生物超过。采用“多重自动化基因组工程”方法，Farren Issacs及同事设计了一系列转基因生物，其生长受到依赖于外源供应的合成氨基酸的必要基因之表达的限制。他们还设计了在所设计的生物与环境之间具有先进“正交屏障”(orthogonal barrier)的合成“营养缺陷体”(auxotroph),从而能够生成更安全的转基因生物。%Genetically modified organisms (GMOs) are increasingly deployed at large scales and in open environments. Genetic biocontainment strategies are needed to prevent unintended proliferation of GMOs in natural ecosystems. Existing biocontainment methods are insufficient because they impose evolutionary pressure on the organism to eject the safeguard by spontaneous mutagenesis or horizontal gene transfer, or because they can be circumvented by environmentally available compounds. Here we computationally redesign essential enzymes in the first organism possessing an altered genetic code (Escherichia coli strain C321.AA) to confer metabolic dependence on non-standard amino acids for survival. The resulting GMOs cannot metabolically bypass their biocontainment mechanisms using known environmental compounds, and they exhibit unprecedented resistance to evolutionary escape through mutagenesis and horizontal gene transfer. This work provides a foundation for safer GMOs that are isolated from natural ecosystems by a reliance on synthetic metabolites.
机译：本期Nature上发表的两篇论文描述了生成一种依赖于非天然氨基酸的生物的独立方法，这一成果在生物封存(biocontainment)和探索以前未曾尝试过的健身方法方面可能会找到应用。George Church及同事通过一个被改变的基因代码对一种生物（大肠杆菌）的必要酶进行了重新设计，以使其在代谢上依赖于非标准氨基酸来存活。这样得到的转基因生物在代谢上无法绕开它们的生物封存机制，并且对演化逃避(evolutionary escape)也表现出前所未有的抵抗力。少数逃避者(escapees)很快被未转基因的生物超过。采用“多重自动化基因组工程”方法，Farren Issacs及同事设计了一系列转基因生物，其生长受到依赖于外源供应的合成氨基酸的必要基因之表达的限制。他们还设计了在所设计的生物与环境之间具有先进“正交屏障”(orthogonal barrier)的合成“营养缺陷体”(auxotroph),从而能够生成更安全的转基因生物。%Genetically modified organisms (GMOs) are increasingly deployed at large scales and in open environments. Genetic biocontainment strategies are needed to prevent unintended proliferation of GMOs in natural ecosystems. Existing biocontainment methods are insufficient because they impose evolutionary pressure on the organism to eject the safeguard by spontaneous mutagenesis or horizontal gene transfer, or because they can be circumvented by environmentally available compounds. Here we computationally redesign essential enzymes in the first organism possessing an altered genetic code (Escherichia coli strain C321.AA) to confer metabolic dependence on non-standard amino acids for survival. The resulting GMOs cannot metabolically bypass their biocontainment mechanisms using known environmental compounds, and they exhibit unprecedented resistance to evolutionary escape through mutagenesis and horizontal gene transfer. This work provides a foundation for safer GMOs that are isolated from natural ecosystems by a reliance on synthetic metabolites.

著录项

  • 来源
    《Nature》 |2015年第7537期|55-60a1|共7页
  • 作者

    Daniel J. Mandell; Marc J. Lajoie; Michael T. Mee; Ryo Takeuchi; Gleb Kuznetsov; Julie E. Norville; Christopher J. Gregg; Barry L. Stoddard; George M. Church;

  • 作者单位

    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA;

    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA,Program in Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA;

    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA,Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, USA;

    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA;

    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA;

    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA;

    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA;

    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA;

    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA,Wyss Institute for Biologically Inspired Engineering. Harvard University, Boston, Massachusetts 02115, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号