Architecture of the large subunit of the mammalian mitochondrial ribosome

Basil J. Greber; Daniel Boehringer; Alexander Leitner; Philipp Bieri; Felix Voigts-Hoffmann; Jan P. Erzberger; Marc Leibundgut; Ruedi Aebersold; Nenad Ban

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Architecture of the large subunit of the mammalian mitochondrial ribosome

机译：哺乳动物线粒体核糖体大亚基的结构

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Mitochondrial ribosomes synthesize a number of highly hydrophobic proteins encoded on the genome of mitochondria, the organelles in eukaryotic cells that are responsible for energy conversion by oxidative phosphorylation. The ribosomes in mammalian mitochondria have undergone massive structural changes throughout their evolution, including ribosomal RNA shortening and acquisition of mitochondria-specific ribosomal proteins. Here we present the three-dimensional structure of the 39S large subunit of the porcine mitochondrial ribosome determined by cryo-electron microscopy at 4.9 A resolution. The structure, combined with data from chemical crosslinking and mass spectrometry experiments, reveals the unique features of the 39S subunit at near-atomic resolution and provides detailed insight into the architecture of the polypeptide exit site. This region of the mitochondrial ribosome has been considerably remodelled compared to its bacterial counterpart, providing a specialized platform for the synthesis and membrane insertion of the highly hydrophobic protein components of the respiratory chain.%通过有氧呼吸驱动真核细胞的线粒体源自原核生物，所含基因组要小得多，其基因组编码若rn干RNA分子和非常有限的一小类线粒体蛋白。哺乳动物细胞的线粒体核糖体的功能非常专 -，是用于呼吸链的膜蛋白翻译的。Nenad Ban及同事通过低温电子显微镜以4.9 A的分辨率获得了较大的39S线粒体核糖体亚单元的三维结构。他们的图像提供了关于这一核糖体内发生的相当大变化的详细信息，这些变化可能是为了帮助由线粒体基因组编码的非常憎水的蛋白的翻译的。

机译：线粒体核糖体合成许多编码在线粒体基因组上的高度疏水性蛋白质，线粒体是真核细胞中的细胞器，负责通过氧化磷酸化进行能量转换。哺乳动物线粒体中的核糖体在整个进化过程中经历了巨大的结构变化，包括核糖体RNA缩短和线粒体特异的核糖体蛋白的获得。在这里，我们介绍了在4.9 A分辨率下通过冷冻电子显微镜确定的猪线粒体核糖体39S大亚基的三维结构。该结构与化学交联和质谱实验的数据相结合，以接近原子的分辨率揭示了39S亚基的独特特征，并提供了对多肽出口位点结构的详细了解。与细菌的对应物相比，线粒体核糖体的这一区域已被重大改造，为呼吸链的高度疏水性蛋白质成分的合成和膜插入提供了专门的平台。原核生物，所含基因组要小得多，其基因组编码若若干RNA分子和非常有限的一小类线粒体蛋白。哺乳动物细胞的线粒体核糖体的功能非常专--是用于呼吸链的膜蛋白。 Nenad Ban及同事通过低温电子显微镜以4.9 A的分辨率获得了透射的39S线粒体核糖体亚单元的三维结构。他们的图像提供了关于这一核糖体内发生的相当大变化的详细信息，这些变化可能是为了帮助由线粒体基因组编码的非常憎水的蛋白质的翻译的。

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来源
《Nature》 |2014年第7484期|515-519A3|共6页
作者
Basil J. Greber; Daniel Boehringer; Alexander Leitner; Philipp Bieri; Felix Voigts-Hoffmann; Jan P. Erzberger; Marc Leibundgut; Ruedi Aebersold; Nenad Ban;
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作者单位

Department of Biology, Institute of Molecular Biology and Biophysics, Schafmattstrasse 20, ETH Zurich, CH-8093 Zurich, Switzerland;

Department of Biology, Institute of Molecular Biology and Biophysics, Schafmattstrasse 20, ETH Zurich, CH-8093 Zurich, Switzerland;

Department of Biology, Institute of Molecular Systems Biology, Wolfgang-Pauli-Strasse 16, ETH Zurich, CH-8093 Zurich, Switzerland;

Department of Biology, Institute of Molecular Biology and Biophysics, Schafmattstrasse 20, ETH Zurich, CH-8093 Zurich, Switzerland;

Department of Biology, Institute of Molecular Biology and Biophysics, Schafmattstrasse 20, ETH Zurich, CH-8093 Zurich, Switzerland;

Department of Biology, Institute of Molecular Biology and Biophysics, Schafmattstrasse 20, ETH Zurich, CH-8093 Zurich, Switzerland;

Department of Biology, Institute of Molecular Biology and Biophysics, Schafmattstrasse 20, ETH Zurich, CH-8093 Zurich, Switzerland;

Department of Biology, Institute of Molecular Systems Biology, Wolfgang-Pauli-Strasse 16, ETH Zurich, CH-8093 Zurich, Switzerland,Faculty of Science, University of Zurich, CH-8057 Zurich, Switzerland;

Department of Biology, Institute of Molecular Biology and Biophysics, Schafmattstrasse 20, ETH Zurich, CH-8093 Zurich, Switzerland;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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专利

1. The complete structure of the large subunit of the mammalian mitochondrial ribosome [J] . Basil J. Greber, Daniel Boehringer, Marc Leibundgut, Nature . 2014,第7526期

机译：哺乳动物线粒体核糖体大亚基的完整结构
2. Methylation of 12S rRNA is necessary for in vivo stability of the small subunit of the mammalian mitochondrial ribosome. [J] . Metodiev MD, Lesko N, Park CB Cell metabolism . 2009,第4期

机译：12S rRNA的甲基化对于哺乳动物线粒体核糖体小亚基的体内稳定性是必需的。
3. A structural model for the large subunit of the mammalian mitochondrial ribosome [J] . Mears JA, Sharma MR, Gutell RR, Journal of Molecular Biology . 2006,第1期

机译：哺乳动物线粒体核糖体大亚基的结构模型
4. Mitochondrial cytochrome c oxidase subunit I sequence variation in New Zealand and overseas specimens of Pieris brassicae (Lepidoptera: Pieridae) [C] . E. Hiszczynska-Sawicka, C.B. Phillips Annual Conferenceof the New Zealand Plant Protection Society s . 2014

机译：线粒体细胞色素C氧化酶亚基I序列变异在Pieris Brassicae新西兰和海外标本（鳞翅目：Pieridae）
5. Cryo-electron microscopy and single particle reconstructions of the Leishmania major ribosome and of the encephalomyocarditis virus internal ribosome entry site bound to the 40S subunit. [D] . Jobe, Amy. 2017

机译：低温电子显微镜和利什曼原虫主要核糖体以及脑心肌炎病毒内部核糖体进入位点结合到40S亚基的单颗粒重建。
6. Correction for Kaushal et al. Cryo-EM structure of the small subunit of the mammalian mitochondrial ribosome [O] . 2015

机译：对Kaushal等人的校正哺乳动物线粒体核糖体小亚基的Cryo-EM结构
7. Correction for Kaushal et al., Cryo-EM structure of the small subunit of the mammalian mitochondrial ribosome [O] . 2015

机译：Kaushal等人的矫正，哺乳动物线粒体核糖体的小亚基的Cryo-EM结构

Architecture of the large subunit of the mammalian mitochondrial ribosome

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