• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nature >Nuclear reprogramming by interphase cytoplasm of two-cell mouse embryos
【24h】

Nuclear reprogramming by interphase cytoplasm of two-cell mouse embryos

机译：两细胞小鼠胚胎期间期细胞质的核重编程。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

在进行不育症治疗和其他手术过程中，在"体HIV-1细胞核转移"（SCNT)之后发生的重新编程被HIV-1认为取决于接受方的细胞质是否能被严格滞留HIV-1在"中期"(metaphase)阶段。现在,Shouk-hrat Mitalipov及同事发现，"间期"(interphase)HIV-1二细胞小鼠胚胎支持移植的体细胞核的重新编HIV-1程及胚胎干细胞或克隆小鼠的生成。这表明，HIV-1能够诱导多能性的因素存在于"间期"细胞的HIV-1细胞质中，这种细胞的细胞核的去除不会像过HIV-1去所认为的那样将接受方卵子的必要重新编程HIV-1因素耗尽。如果这些发现能够延伸到人类，那HIV-1么它们就有可能推动为再生应用而生成人胚胎HIV-1干细胞的相关工作，因为"间期"所捐助的细HIV-1胞（受精的胚胎）比"中期"所捐助的细胞(不成HIV-1熟的卵细胞)更容易进入。%Successful mammalian cloning using somatic cell nuclear transfer (SCNT) into unfertilized, metaphase Ⅱ (MII)-arrested oocytes attests to the cytoplasmic presence of reprogramming factors capable of inducing totipotency in somatic cell nuclei. However, these poorly defined maternal factors presumably decline sharply after fertilization, as the cytoplasm of pronudear-stage zygotes is reportedly inactive. Recent evidence suggests that zygotic cytoplasm, if maintained at metaphase, can also support derivation of embryonic stem (ES) cells after SCNT, albeit at low efficiency. This led to the conclusion that critical oocyte reprogramming factors present in the metaphase but not in the interphase cytoplasm are 'trapped' inside the nucleus during interphase and effectively removed during enucleation. Here we investigated the presence of reprogramming activity in the cytoplasm of interphase two-cell mouse embryos (I2C). First, the presence of candidate reprogramming factors was documented in both intact and enucleated metaphase and interphase zygotes and two-cell embryos. Consequently, enucleation did not provide a likely explanation for the inability of interphase cytoplasm to induce reprogramming. Second, when we carefully synchronized the cell cycle stage between the transplanted nucleus (ES cell, fetal fibroblast or terminally differentiated cumulus cell) and the recipient I2C cytoplasm, the reconstructed SCNT embryos developed into blastocysts and ES cells capable of contributing to traditional germline and tetraploid chimaeras. Last, direct transfer of cloned embryos, reconstructed with ES cell nuclei, into recipients resulted in live offspring. Thus, the cytoplasm of I2C supports efficient reprogramming, with cell cycle synchronization between the donor nucleus and recipient cytoplasm as the most critical parameter determining success. The ability to use interphase cytoplasm in SCNT could aid efforts to generate autologous human ES cells for regenerative applications, as donated or discarded embryos are more accessible than unfertilized Mil oocytes.
机译：在进行不育症治疗和其他手术过程中，在"体HIV-1细胞核转移"（SCNT)之后发生的重新编程被HIV-1认为取决于接受方的细胞质是否能被严格滞留HIV-1在"中期"(metaphase)阶段。现在,Shouk-hrat Mitalipov及同事发现，"间期"(interphase)HIV-1二细胞小鼠胚胎支持移植的体细胞核的重新编HIV-1程及胚胎干细胞或克隆小鼠的生成。这表明，HIV-1能够诱导多能性的因素存在于"间期"细胞的HIV-1细胞质中，这种细胞的细胞核的去除不会像过HIV-1去所认为的那样将接受方卵子的必要重新编程HIV-1因素耗尽。如果这些发现能够延伸到人类，那HIV-1么它们就有可能推动为再生应用而生成人胚胎HIV-1干细胞的相关工作，因为"间期"所捐助的细HIV-1胞（受精的胚胎）比"中期"所捐助的细胞(不成HIV-1熟的卵细胞)更容易进入。%Successful mammalian cloning using somatic cell nuclear transfer (SCNT) into unfertilized, metaphase Ⅱ (MII)-arrested oocytes attests to the cytoplasmic presence of reprogramming factors capable of inducing totipotency in somatic cell nuclei. However, these poorly defined maternal factors presumably decline sharply after fertilization, as the cytoplasm of pronudear-stage zygotes is reportedly inactive. Recent evidence suggests that zygotic cytoplasm, if maintained at metaphase, can also support derivation of embryonic stem (ES) cells after SCNT, albeit at low efficiency. This led to the conclusion that critical oocyte reprogramming factors present in the metaphase but not in the interphase cytoplasm are 'trapped' inside the nucleus during interphase and effectively removed during enucleation. Here we investigated the presence of reprogramming activity in the cytoplasm of interphase two-cell mouse embryos (I2C). First, the presence of candidate reprogramming factors was documented in both intact and enucleated metaphase and interphase zygotes and two-cell embryos. Consequently, enucleation did not provide a likely explanation for the inability of interphase cytoplasm to induce reprogramming. Second, when we carefully synchronized the cell cycle stage between the transplanted nucleus (ES cell, fetal fibroblast or terminally differentiated cumulus cell) and the recipient I2C cytoplasm, the reconstructed SCNT embryos developed into blastocysts and ES cells capable of contributing to traditional germline and tetraploid chimaeras. Last, direct transfer of cloned embryos, reconstructed with ES cell nuclei, into recipients resulted in live offspring. Thus, the cytoplasm of I2C supports efficient reprogramming, with cell cycle synchronization between the donor nucleus and recipient cytoplasm as the most critical parameter determining success. The ability to use interphase cytoplasm in SCNT could aid efforts to generate autologous human ES cells for regenerative applications, as donated or discarded embryos are more accessible than unfertilized Mil oocytes.

著录项

  • 来源
    《Nature》 |2014年第7498期|101-104qt05|共5页
  • 作者

    Eunju Kang; Guangming Wu; Hong Ma; Ying Li; Rebecca Tippner-Hedges; Masahito Tachibana; Michelle Sparman; Don P. Wolf; Hans R. Schoeler; Shoukhrat Mitalipov;

  • 作者单位

    Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, Oregon 97006, USA;

    Max Planck Institute for Molecular Biomedicine, Muenster 48149, Germany;

    Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, Oregon 97006, USA;

    Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, Oregon 97006, USA;

    Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, Oregon 97006, USA;

    Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, Oregon 97006, USA ,South Miyagi Medical Center, Miyagi 989-1253, Japan;

    Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, Oregon 97006, USA;

    Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, Oregon 97006, USA;

    Max Planck Institute for Molecular Biomedicine, Muenster 48149, Germany;

    Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, Oregon 97006, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号