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Accurate design of co- assembling multi-component protein nanomaterials

机译:共组装多组分蛋白质纳米材料的精确设计

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摘要

The self-assembly of proteins into highly ordered nanoscale architectures is a hallmark of biological systems. The sophisticated functions of these molecular machines have inspired the development of methods to engineer self-assembling protein nanostructures; however, the design of multi-component protein nanomaterials with high accuracy remains an outstanding challenge. Here we report a computational method for designing protein nanomaterials in which multiple copies of two distinct subunits co-assemble into a specific architecture. We use the method to design five 24-subunit cage-like protein nanomaterials in two distinct symmetric architectures and experimentally demonstrate that their structures are in close agreement with the computational design models. The accuracy of the method and the number and variety of two - component materials that it makes accessible suggest a route to the construction of functional protein nanomaterials tailored to specific applications.
机译:蛋白质自组装成高度有序的纳米级结构是生物系统的标志。这些分子机器的复杂功能激发了开发工程技术以自我组装蛋白质纳米结构的方法。然而,高精度的多组分蛋白质纳米材料的设计仍然是一个巨大的挑战。在这里,我们报告了一种设计蛋白质纳米材料的计算方法,其中两个不同亚基的多个副本共同组装成一个特定的体系结构。我们使用该方法在两种不同的对称结构中设计了五种24个亚基笼状蛋白纳米材料,并通过实验证明了它们的结构与计算设计模型非常吻合。该方法的准确性以及可获取的两组分材料的数量和种类,为构建针对特定应用的功能蛋白纳米材料提供了一条途径。

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  • 来源
    《Nature》 |2014年第7503期|103-108|共6页
  • 作者单位

    Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA,Institute for Protein Design, University of Washington, Seattle, Washington 98195, USA;

    Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA,Graduate Program in Molecular and Cellular Biology, University of Washington, Seattle, Washington 98195, USA;

    Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA;

    UCLA Department of Chemistry and Biochemistry, Los Angeles, California 90095, USA;

    Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA,Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia 20147, USA;

    Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia 20147, USA;

    UCLA Department of Chemistry and Biochemistry, Los Angeles, California 90095, USA,UCLA-D0E Institute for Genomics and Proteomics, Los Angeles, California 90095, USA,UCLA Molecular Biology Institute, Los Angeles, California 90095, USA;

    Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA,Institute for Protein Design, University of Washington, Seattle, Washington 98195, USA,Howard Hughes Medical Institute, University of Washington, Seattle, Washington 98195, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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