Wobble puts RNA on target

OSCAR VARGAS-RODRIGUEZ; KARIN MUSIER-FORSYTH

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Wobble puts RNA on target

机译：摆动将RNA置于靶标上

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How genetic information encoded in nucleic acids is translated into amino-acid sequences in proteins has fascinated researchers for decades. Faithful translation involves specific pairing of triplets of nucleotides, called codons, in messenger RNA with complementary anticodons in transfer RNA. High accuracy also requires the attachment (aminoacylation) of each type of amino acid to a specific type of tRNA. Each variant of the aminoacylation reaction is catalysed by a particular aminoacyl-tRNA synthetase (ARS) enzyme, which must distinguish its own amino-acid substrate and the matching tRNA from the large pool of similar substrates. In this issue, Naganuma et al (page 507) report crystal structures that cast light on the remarkable mechanism by which information encoded in part of a tRNA is interpreted by an ARS, resulting in highly specific aminoacylation. One of the simplest examples of tRNA recognition is that of alanyl-tRNA synthetase (AlaRS), the enzyme that attaches the amino acid alanine to its tRNA (tRNA~(Ala)). Across all domains of life, this recognition process relies primarily on a single 'wobble' base pair (designated G3-U70, where G and U represent the bases guanine and uracil, respectively) in a region of tRNA~(Ala) called the acceptor stem.

机译：数十年来，研究人员着迷于如何将核酸编码的遗传信息转化为蛋白质中的氨基酸序列。忠实的翻译涉及信使RNA中的三重核苷酸（称为密码子）与转移RNA中的互补反密码子的特定配对。高精度还需要将每种类型的氨基酸附着（氨基酰化）到特定类型的tRNA。氨基酰化反应的每个变体均由特定的氨酰基tRNA合成酶（ARS）催化，该酶必须将自己的氨基酸底物和匹配的tRNA与大量相似的底物区分开来。在此期中，Naganuma等人（第507页）报告了晶体结构，这种晶体结构将亮点引人注目的机制，即通过aRS解释tRNA部分编码信息的显着机理，从而导致高度特异性的氨酰化作用。 tRNA识别的最简单例子之一是丙氨酰-tRNA合成酶（AlaRS），它是将氨基酸丙氨酸连接到其tRNA的酶（tRNA〜（Ala））。在整个生活领域中，这种识别过程主要依赖于称为受体的tRNA〜（Ala）区域中的单个“摆动”碱基对（命名为G3-U70，其中G和U分别代表鸟嘌呤和尿嘧啶的碱基）。干。

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来源
《Nature》 |2014年第7506期|480-481|共2页
作者
OSCAR VARGAS-RODRIGUEZ; KARIN MUSIER-FORSYTH;
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作者单位

Department of Chemistry and Biochemistry, Center for RNA Biology, Ohio State University, Columbus, Ohio 43210, USA;

Department of Chemistry and Biochemistry, Center for RNA Biology, Ohio State University, Columbus, Ohio 43210, USA;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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专利

1. Effective suppression of HIV-1 by artificial bispecific miRNA targeting conserved sequences with tolerance for wobble base-pairing. [J] . Son J, Uchil PD, Kim YB, Biochemical and Biophysical Research Communications . 2008,第2期

机译：人工双特异性miRNA靶向保守序列对摆动碱基配对具有耐受性，可有效抑制HIV-1。
2. A single uridine modification at the wobble position of an artificial tRNA enhances wobbling in an Escherichia coli cell‐free translation system [J] . Hosono Kazumi, Okumura Shuhei, Takai Kazuyuki, FEBS Letters . 1999,第1期

机译：人工tRNA摆动位置的单个尿苷修饰可增强大肠杆菌无细胞翻译系统中的摆动
3. Stacking of Crick Wobble pair and Watson-Crick pair: stability rules of G-U pairs at ends of helical stems in tRNAs and the relation to codon-anticodon Wobble interaction [J] . H. Mizuno, M. Sundaralingam Nucleic acids research . 1978,第11期

机译：Crick摆动对和Watson-Crick对的堆叠：tRNA螺旋茎末端的G-U对的稳定性规则及其与密码子-反密码子相互作用的关系
4. Selenocysteine (Sec) tRNASerSec And Selenoprotein Levels In Transgenic Mice Expressing Sec tRNASerSec With A Mutation In The Wobble Position [C] . 19th tRNA workshop tRNA 2002"" . 2002

机译：摆动位点突变的表达Sec tRNA Ser Sec的转基因小鼠中的硒代半胱氨酸（Sec）tRNA Ser Sec和硒蛋白水平
5. Crystal structures of alternating RNA oligomers: Tandem G-U wobble pairs [D] . Biswas, Roopa 1997

机译：交替的RNA低聚物的晶体结构：串联G-U摆动对
6. Stacking of Crick Wobble pair and Watson-Crick pair: stability rules of G-U pairs at ends of helical stems in tRNAs and the relation to codon-anticodon Wobble interaction. [O] . H Mizuno, M Sundaralingam 1978

机译：Crick摆动对和Watson-Crick对的堆叠：tRNA螺旋茎末端的G-U对的稳定性规则以及与密码子-反密码子相互作用的关系。
7. A single uridine modification at the wobble position of an artificial tRNA enhances wobbling in an Escherichia coli cell-free translation system [O] . Takai Kazuyuki, Okumura Shuhei, Hosono Kazumi, 1999

机译：人工tRNA摆动位置的单个尿苷修饰可增强大肠杆菌无细胞翻译系统中的摆动

Wobble puts RNA on target

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