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Mitotic trigger waves and the spatial coordination of the Xenopus cell cycle

机译：有丝分裂触发波与非洲爪蟾细胞周期的空间协调

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摘要

Despite the large size of the Xenopus laevis egg (approximately 1.2 mm diameter), a fertilized egg rapidly proceeds through mitosis in a spatially coordinated fashion. Mitosis is initiated by a bistable system of regulatory proteins centred on Cdkl (refs 1,2), raising the possibility that this spatial coordination could be achieved through trigger waves of Cdkl activity. Using an extract system that performs cell cycles in vitroy here we show that mitosis does spread through Xenopus cytoplasm via trigger waves, propagating at a linear speed of approximately 60μm min~(-1). Perturbing the feedback loops that give rise to the bistability of Cdkl changes the speed and dynamics of the waves. Time-lapse imaging of intact eggs argues that trigger waves of Cdkl activation are responsible for surface contraction waves, ripples in the cell cortex that precede cytokinesis. These findings indicate that Cdkl trigger waves help ensure the spatiotemporal coordination of mitosis in large eggs. Trigger waves may be an important general mechanism for coordinating biochemical events over large distances.
机译：尽管非洲爪蟾卵的尺寸很大（直径约1.2毫米），但受精卵以空间协调的方式迅速通过有丝分裂。有丝分裂是由以Cdkl为中心的双稳态调节蛋白系统启动的（参考文献1,2），这增加了通过Cdkl活性触发波实现这种空间协调的可能性。在这里，我们使用提取物系统在体外进行细胞周期研究，结果表明有丝分裂确实通过触发波在非洲爪蟾细胞质中传播，并以约60μmmin〜（-1）的线速度传播。扰动引起Cdkl双稳态的反馈回路会改变波的速度和动力学。完整卵的延时成像表明，Cdkl激活的触发波是表面收缩波的原因，表面收缩波是胞质分裂之前细胞皮质中的波纹。这些发现表明，Cdkl触发波有助于确保大卵中有丝分裂的时空协调。触发波可能是协调长距离生化事件的重要通用机制。

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  • 来源
    《Nature》 |2013年第7464期|603-607|共5页
  • 作者

    Jeremy B. Chang; James E. Ferrell Jr;

  • 作者单位

    Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford. California 94305-5174, USA;

    Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford. California 94305-5174, USA,Department of Biochemistry. Stanford University School of Medicine,Stanford, California 94305-5307, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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