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Structure of a methyl-coenzyme M reductase from Black Sea mats that oxidize methane anaerobically

机译：来自黑海垫的甲基辅酶M还原酶的结构，其厌氧氧化甲烷

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摘要

The anaerobic oxidation of methane (AOM) with sulphate, an area currently generating great interest in microbiology, is accomplished by consortia of methanotrophic archaea (ANME) and sulphate-reducing bacteria1'2. The enzyme activating methane in methanotrophic archaea has tentatively been identified as a homologue of methyl-coenzyme M reductase (MCR) that catalyses the methane-forming step in methanogenic archaea3'4. Here we report an X-ray structure of the 280 kDa heterohexameric ANME-1 MCR complex. It was crystallized uniquely from a protein ensemble purified from consortia of microorganisms collected with a submersible from a Black Sea mat catalysing AOM with sulphate4. Crystals grown from the heterogeneous sample diffract to 2.1 A resolution and consist of a single ANME-1 MCR population, demonstrating the strong selective power of crystallization. The structure revealed ANME-1 MCR in complex with coenzyme M and coenzyme B, indicating the same substrates for MCR from methanotrophic and methanogenic archaea. Differences between the highly similar structures of ANME-1 MCR and methanogenic MCR include a F430 modification, a cysteine-rich patch and an altered post-translational amino acid modification pattern, which may tune the enzymes for their functions in different biological contexts.
机译：甲烷（AOM）的厌氧氧化是目前在微生物学上引起极大兴趣的领域，是由甲烷营养细菌（ANME）和硫酸盐还原细菌1'2组成的。甲烷营养古细菌中激活甲烷的酶已被初步鉴定为甲基辅酶M还原酶（MCR）的同系物，该酶催化产甲烷古菌中甲烷形成步骤3'4。在这里，我们报告了280 kDa异六聚体ANME-1 MCR复合物的X射线结构。它是从蛋白质集合体中唯一结晶的，该蛋白质集合体是从用硫酸盐催化AOM的黑海垫中潜水的潜水器收集的微生物菌群中纯化的。从异质样品中生长出来的晶体衍射至2.1 A分辨率，并由单个ANME-1 MCR族组成，证明了强大的结晶选择性。该结构揭示了ANME-1 MCR与辅酶M和辅酶B的复合物，表明来自甲烷营养和产甲烷古菌的MCR底物相同。 ANME-1 MCR和产甲烷MCR的高度相似结构之间的差异包括F430修饰，富含半胱氨酸的贴片和改变的翻译后氨基酸修饰模式，这可能会调节酶在不同生物学环境下的功能。

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  • 来源
    《Nature》 |2012年第7379期|p.98-101|共4页
  • 作者

    Seigo Shima; Martin Krueger; Tobias Weinert; Ulrike Demmer; Jorg Kahnt; Rudolf K. Thauer; Ulrich Ermler;

  • 作者单位

    Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse 10, D-35043 Marburg, Germany,PREST0, Japan Science and Technology Agency (JST), Honcho, Kawaguchi.Saitama 332-0012 Japan;

    Federal Institute for Geosciences and Resources, Stilleweg 2,30655 Hannover, Germany;

    Max Planck Institute for Biophysics, Max-von-Laue-Strasse 3, D-60438 Frankfurt, Germany;

    Max Planck Institute for Biophysics, Max-von-Laue-Strasse 3, D-60438 Frankfurt, Germany;

    Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse 10, D-35043 Marburg, Germany;

    Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse 10, D-35043 Marburg, Germany;

    Max Planck Institute for Biophysics, Max-von-Laue-Strasse 3, D-60438 Frankfurt, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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