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Preferential electrical coupling regulates neocortical lineage-dependent microcircuit assembly

机译：优先电耦合调节新皮层谱系依赖性微电路组装

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摘要

人们曾提出，在发育过程中，在克隆上相关的rn神经元会沿同一辐射状神经胶质纤维迁移，形rn成由在功能上相似的细胞所构成的团簇。然rn而，这种现象一直没有在实验中发现。现在，rn两个小组演示了决定随后的功能关系的发育中的大脑皮层中的姐妹神经元之间的电耦合。rnSon-Hai Shi及其同事报告，在来自出生后小rn鼠的新皮层组织中，姐妹神经元之间的长距离rn连接是通过在它们之间的化学突触形成之前发rn生的电耦合来维持的。发育过程中间隙连接的rn任何阻断，都会中断姐妹细胞最终的突触连rn接，阻止它们的同步放电。Yang Dan及其同rn事发现，在小鼠视觉皮层中，同一辐射克隆中rn的姐妹神经元有相似的取向偏好。在出生后很rn短时间内中断间隙连接耦合，会降低姐妹神经rn元之间的功能相似性，说明来自在个体发育方rn面相关的神经元的功能性组织的形成需要这种rn形式的神经通信。%Radial glial cells are the primary neural progenitor cells in the developing neocortex. Consecutive asymmetric divisions of individual radial glial progenitor cells produce a number of sister excitatory neurons that migrate along the elongated radial glial fibre, resulting in the formation of ontogenetic columns. Moreover, sister excitatory neurons in ontogenetic columns preferentially develop specific chemical synapses with each other rather than with nearby non-siblings. Although these findings provide crucial insight into the emergence of functional columns in the neocortex, little is known about the basis of this lineage-dependent assembly of excitatory neuron microdrcuits at single-cell resolution. Here we show that transient electrical coupling between radially aligned sister excitatory neurons regulates the subsequent formation of specific chemical synapses in the neocortex. Multiple-electrode whole-cell recordings showed that sister excitatory neurons preferentially form strong electrical coupling with each other rather than with adjacent non-sister excitatory neurons during early postnatal stages. This preferential coupling allows selective electrical communication between sister excitatory neurons, promoting their action potential generation and synchronous firing. Interestingly, although this electrical communication largely disappears before the appearance of chemical synapses, blockade of the electrical communication impairs the subsequent formation of specific chemical synapses between sister excitatory neurons in ontogenetic columns. These results suggest a strong link between lineage-dependent transient electrical coupling and the assembly of precise excitatory neuron microcircuits in the neocortex.
机译：人们曾提出，在发育过程中，在克隆上相关的rn神经元会沿同一辐射状神经胶质纤维迁移，形rn成由在功能上相似的细胞所构成的团簇。然rn而，这种现象一直没有在实验中发现。现在，rn两个小组演示了决定随后的功能关系的发育中的大脑皮层中的姐妹神经元之间的电耦合。rnSon-Hai Shi及其同事报告，在来自出生后小rn鼠的新皮层组织中，姐妹神经元之间的长距离rn连接是通过在它们之间的化学突触形成之前发rn生的电耦合来维持的。发育过程中间隙连接的rn任何阻断，都会中断姐妹细胞最终的突触连rn接，阻止它们的同步放电。Yang Dan及其同rn事发现，在小鼠视觉皮层中，同一辐射克隆中rn的姐妹神经元有相似的取向偏好。在出生后很rn短时间内中断间隙连接耦合，会降低姐妹神经rn元之间的功能相似性，说明来自在个体发育方rn面相关的神经元的功能性组织的形成需要这种rn形式的神经通信。%Radial glial cells are the primary neural progenitor cells in the developing neocortex. Consecutive asymmetric divisions of individual radial glial progenitor cells produce a number of sister excitatory neurons that migrate along the elongated radial glial fibre, resulting in the formation of ontogenetic columns. Moreover, sister excitatory neurons in ontogenetic columns preferentially develop specific chemical synapses with each other rather than with nearby non-siblings. Although these findings provide crucial insight into the emergence of functional columns in the neocortex, little is known about the basis of this lineage-dependent assembly of excitatory neuron microdrcuits at single-cell resolution. Here we show that transient electrical coupling between radially aligned sister excitatory neurons regulates the subsequent formation of specific chemical synapses in the neocortex. Multiple-electrode whole-cell recordings showed that sister excitatory neurons preferentially form strong electrical coupling with each other rather than with adjacent non-sister excitatory neurons during early postnatal stages. This preferential coupling allows selective electrical communication between sister excitatory neurons, promoting their action potential generation and synchronous firing. Interestingly, although this electrical communication largely disappears before the appearance of chemical synapses, blockade of the electrical communication impairs the subsequent formation of specific chemical synapses between sister excitatory neurons in ontogenetic columns. These results suggest a strong link between lineage-dependent transient electrical coupling and the assembly of precise excitatory neuron microcircuits in the neocortex.

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  • 来源
    《Nature》 |2012年第7401期|p.113-117A1A3|共7页
  • 作者

    Yong-Chun Yu; Shuijin He; She Chen; Yinghui Fu; Keith N. Brown; Xing-Hua Yao; Jian Ma; Kate P. Gao; Gina E. Sosinsky; Kun Huang; Song-Hai Shi;

  • 作者单位

    Institute of Neurobiology, Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China;

    Developmental Biology Program, Memorial Sloan-KetteringCancer Centre, 1275 York Avenue, New York, New York 10065, USA;

    Developmental Biology Program, Memorial Sloan-KetteringCancer Centre, 1275 York Avenue, New York, New York 10065, USA;

    Institute of Neurobiology, Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China;

    Developmental Biology Program, Memorial Sloan-KetteringCancer Centre, 1275 York Avenue, New York, New York 10065, USA,Neuroscience Graduate Program, Weill Cornell Medical College, 1230 York Avenue, New York, New York 10065, USA;

    Institute of Neurobiology, Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China;

    Institute of Neurobiology, Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China;

    Developmental Biology Program, Memorial Sloan-KetteringCancer Centre, 1275 York Avenue, New York, New York 10065, USA,Neuroscience Graduate Program, Weill Cornell Medical College, 1230 York Avenue, New York, New York 10065, USA;

    National Centre for Microscopy and Imaging Research and Department of Neurosciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0608, USA.;

    Department of Biomedical Informatics, Comprehensive Cancer Center Biomedical Informatics Shared Resource, The Ohio State University, 333 West 10th Avenue, Columbus, Ohio 43210, USA.;

    Developmental Biology Program, Memorial Sloan-KetteringCancer Centre, 1275 York Avenue, New York, New York 10065, USA,Neuroscience Graduate Program, Weill Cornell Medical College, 1230 York Avenue, New York, New York 10065, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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