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Crystal structure of a phosphorylation-coupled saccharide transporter

机译:磷酸化偶联糖转运蛋白的晶体结构

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摘要

Saccharides have a central role in the nutrition of all living organisms. Whereas several saccharide uptake systems are shared between the different phylogenetic kingdoms, the phosphoenolpyruvate-dependent phosphotransferase system exists almost exclusively in bacteria. This multi-component system includes an integral membrane protein EIIC that transports saccharides and assists in their phosphorylation. Here we present the crystal structure of an EIIC from Bacillus cereus that transports diacetylchitobiose. The EIIC is a homodimer, with an expansive interface formed between the amino-terminal halves of the two protomers. The carboxy-terminal half of each protomer has a large binding pocket that contains a diacetylchitobiose, which is occluded from both sides of the membrane with its site of phosphorylation near the conserved His 250 and Glu 334 residues. The structure shows the architecture of this important class of transporters, identifies the determinants of substrate binding and phosphorylation, and provides a framework for understanding the mechanism of sugar translocation.
机译:糖在所有活生物体的营养中具有重要作用。尽管在不同的系统发育王国之间共有几个糖分摄取系统,但是依赖磷酸烯醇丙酮酸的磷酸转移酶系统几乎完全存在于细菌中。该多组分系统包括一个完整的膜蛋白EIIC,该蛋白可转运糖并帮助其磷酸化。在这里,我们介绍了蜡状芽孢杆菌运输二乙酰壳二糖的EIIC的晶体结构。 EIIC是同型二聚体,在两个protomer的氨基末端两半之间形成扩展界面。每个启动子的羧基末端一半都有一个较大的结合口袋,其中含有二乙酰基壳二糖,该二乙酰基壳二糖从膜的两侧闭塞,其磷酸化位点靠近保守的His 250和Glu 334残基。该结构显示了这一重要转运蛋白的体系结构,确定了底物结合和磷酸化的决定因素,并为理解糖转运的机理提供了框架。

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  • 来源
    《Nature》 |2011年第7345期|p.50-54|共5页
  • 作者单位

    Department of Physiology & Cellular Biophysics, College of Physiciansand Surgeons, Columbia University, 630 West 168th Street, New York, New York 10032, USA;

    Center for Computational Biology and Bioinformatics, Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University, 1130 St. Nicholas Ave, Room 815, New York, New York 10032, USA;

    Department of Physiology & Cellular Biophysics, College of Physiciansand Surgeons, Columbia University, 630 West 168th Street, New York, New York 10032, USA;

    Department of Physiology & Cellular Biophysics, College of Physiciansand Surgeons, Columbia University, 630 West 168th Street, New York, New York 10032, USA;

    Sanford-Bumham Institute, La Jolla, California 92037, USA;

    Department of Psychiatry and Center for Molecular Recognition, Columbia University, 630 West 168th Street, New York, New York 10032,USA,New York State Psychiatric Institute, Division of Molecular Therapeutics, 1051 Riverside Drive, New York, New York 10032, USA;

    Department of Physiology & Cellular Biophysics, College of Physiciansand Surgeons, Columbia University, 630 West 168th Street, New York, New York 10032, USA;

    Department of Physiology & Cellular Biophysics, College of Physiciansand Surgeons, Columbia University, 630 West 168th Street, New York, New York 10032, USA;

    New York Consortium on Membrane Protein Structure, New York Structural Biology Center, 89 Convent Avenue, New York, New York 10027, USA;

    New York Consortium on Membrane Protein Structure, New York Structural Biology Center, 89 Convent Avenue, New York, New York 10027, USA,Department of Computer Science and institute for Advanced Study, Technical University of Munich, D-85748 Munich,Germany;

    New York Consortium on Membrane Protein Structure, New York Structural Biology Center, 89 Convent Avenue, New York, New York 10027, USA,Department of Computer Science and institute for Advanced Study, Technical University of Munich, D-85748 Munich,Germany;

    New York Consortium on Membrane Protein Structure, New York Structural Biology Center, 89 Convent Avenue, New York, New York 10027, USA,Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University, 630 West 168th Street, New York, New York 10032, USA;

    Department of Psychiatry and Center for Molecular Recognition, Columbia University, 630 West 168th Street, New York, New York 10032,USA,New York State Psychiatric Institute, Division of Molecular Therapeutics, 1051 Riverside Drive, New York, New York 10032, USA,Department of Pharmacology, Columbia University, 630 West 168th Street, New York, New York 10032, USA;

    Department of Physiology & Cellular Biophysics, College of Physiciansand Surgeons, Columbia University, 630 West 168th Street, New York, New York 10032, USA;

    Department of Physiology & Cellular Biophysics, College of Physiciansand Surgeons, Columbia University, 630 West 168th Street, New York, New York 10032, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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