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Condensin association with histone H2A shapes mitotic chromosomes

机译：凝集素与组蛋白H2A的结合塑造有丝分裂染色体

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摘要

Chromosome structure is dynamically regulated during cell division, and this regulation is dependent, in part, on condensin. The localization of condensin at chromosome arms is crucial for chromosome partitioning during anaphase. Condensin is also enriched at kinetochores but its precise role and loading machinery remain unclear. Here we show that fission yeast (Schizosacckaromyces pombe) kinetochore proteins Pcsl and Mde4—homologues of budding yeast (Saccharomyees cerevisiae) monopolin subunits and known to prevent merotelic kinetochore orientation—act as a condensin 'recruiter' at kinetochores, and that condensin itself may act to clamp microtubule binding sites during metaphase. In addition to the regional recruitment factors, overall condensin association with chromatin is governed by the chromosomal passenger kinase Aurora B. Aurora-B-dependent phosphorylation of condensin promotes its association with histone H2A and H2A.Z, which we identify as conserved chromatin 'receptors' of condensin. Condensin phosphorylation and its deposition onto chromosome arms reach a peak during anaphase, when Aurora B kinase relocates from centromeres to the spindle midzone, where the separating chromosome arms are positioned. Our results elucidate the molecular basis for the spatiotemporal regulation of mitotic chromosome architecture, which is crucial for chromosome partitioning.%在有丝分裂过程中，染色体被凝缩蛋白的作用 变得紧凑和僵硬。这种蛋白被认为在染色体臂 中和在“着丝点”上有不同作用，但它是怎样 定位到这些区域的却不清楚。Tada等人发 现，名为“monopolin”的“着丝点蛋白”是“着 丝点”凝缩蛋白的一个招募者(recruiter)。
机译：染色体结构在细胞分裂过程中受到动态调节，这种调节部分取决于缩合蛋白。凝集素在染色体臂的定位对于后期分裂染色体至关重要。缩合蛋白在动植物中也富集，但其确切作用和装载机制仍不清楚。在这里，我们显示裂变酵母（Schizosacckaromyces pombe）线粒体蛋白Pcsl和Mde4-萌芽酵母（Saccharomyees cerevisiae）单花粉蛋白亚基的同源物，已知能够阻止线粒体线粒体定向-充当凝集素的“征募者”，可能在动植物体内起作用，并且在中期期间夹住微管结合位点。除区域募集因素外，总凝缩蛋白与染色质的结合还受染色体乘客激酶Aurora B的支配。凝视素的Aurora-B依赖性磷酸化促进了其与组蛋白H2A和H2A.Z的结合，我们将其鉴定为保守的染色质'受体凝聚素。当Aurora B激酶从着丝粒重新定位到纺锤体中区时，凝集素的磷酸化及其在染色体臂上的沉积在后期达到一个峰值。我们的结果阐明了有丝分裂染色体结构时空调节的分子基础，这对染色体分区至关重要。％在有丝分裂过程中，染色体被凝缩蛋白的作用变得越来越僵硬。这种蛋白被认为在染色体臂中和在“着丝点”上有不同作用，但它是怎样定位到这些区域的却清晰。Tada等人发现，称为“ monopolin”的“着丝点蛋白”是“着丝点”凝缩蛋白的一个招募者（recruiter）。

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  • 来源
    《Nature》 |2011年第7352期|p.477-483407|共8页
  • 作者

    Kenji Tada; Hiroaki Susumu; Takeshi Sakuno; Yoshinori Watanabe;

  • 作者单位

    Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Tokyo 113-0032, Japan Graduate Program in Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Yayoi, Tokyo 113-0032, Japan;

    Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Tokyo 113-0032, Japan Graduate Program in Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Yayoi, Tokyo 113-0032, Japan;

    Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Tokyo 113-0032, Japan Promotion of Independence for Young Investigators, University of Tokyo, Yayoi, Tokyo 113-0032, Japan;

    Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Tokyo 113-0032, Japan Graduate Program in Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Yayoi, Tokyo 113-0032, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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