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Functional mapping of single spines in cortical neurons in vivo

机译：体内皮层神经元中单个棘的功能图

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摘要

The individual functional properties and spatial arrangement of afferent synaptic inputs on dendrites have a critical role in the processing of information by neurons in the mammalian brain~(1-4). Although recent work has identified visually-evoked local dendritic calcium signals in the rodent visual cortex~5, sensory-evoked signalling on the level of dendritic spines, corresponding to individual afferent excitatory synapses, remains unexplored6. Here we used a new variant of high-resolution two-photon imaging~7 to detect sensory-evoked calcium transients in single dendritic spines of mouse cortical neurons in vivo. Calcium signals evoked by sound stimulation required the activation of NMDA (N-methyl-D-aspartate) receptors. Active spines are widely distributed on basal and apical dendrites and pure-tone stimulation at different frequencies revealed both narrowly and widely tuned spines. Notably, spines tuned for different frequencies were highly interspersed on the same dendrites: even neighbouring spines were mostly tuned to different frequencies. Thus, our results demonstrate that NMD A-receptor-dependent single-spine synaptic inputs to the same dendrite are highly heterogeneous. Furthermore, our study opens the way for in vivo mapping of functionally defined afferent sensory inputs with single-synapse resolution.
机译：树突上传入突触输入的个体功能特性和空间排列在哺乳动物脑中神经元对信息的处理中具有关键作用[1-4]。尽管最近的工作已经在啮齿动物的视觉皮层中发现了视觉诱发的局部树突状钙信号，但仍未探索到树突棘水平的感觉诱发信号，该信号对应于单个传入的兴奋性突触6。在这里，我们使用高分辨率的双光子成像〜7的新变体，在体内检测小鼠皮质神经元的单个树突棘中的感觉诱发的钙瞬变。声音刺激引起的钙信号需要激活NMDA（N-甲基-D-天冬氨酸）受体。活跃的刺广泛分布在基部和顶端的树突上，纯音刺激以不同的频率显示出狭窄和广泛调谐的刺。值得注意的是，针对不同频率调谐的棘突高度散布在同一树突上：即使相邻的棘突也大多被调谐至不同频率。因此，我们的结果表明，向同一树突的NMD A受体依赖性单棘突触输入是高度异质的。此外，我们的研究为功能定义的具有单个突触分辨率的传入感觉输入的体内作图开辟了道路。

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  • 来源
    《Nature》 |2011年第7357期|p.501-505|共5页
  • 作者

    Xiaowei Chen; Ulrich Leischner; Nathalie L. Rochefort; Israel Nelken; Arthur Konnerth;

  • 作者单位

    Institute of Neuroscienceand Center for Integrated Protein Science, Technical University Munich, Biedersteinerstrasse 29,80802 Munich, Germany;

    Institute of Neuroscienceand Center for Integrated Protein Science, Technical University Munich, Biedersteinerstrasse 29,80802 Munich, Germany;

    Institute of Neuroscienceand Center for Integrated Protein Science, Technical University Munich, Biedersteinerstrasse 29,80802 Munich, Germany;

    Department of Neurobiology, Silberman Institute of Life Sciences and the Edmond and Lily Safra Center for Brain Sciences, Hebrew University, Jerusalem 91904, Israel;

    Institute of Neuroscienceand Center for Integrated Protein Science, Technical University Munich, Biedersteinerstrasse 29,80802 Munich, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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