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首页> 外文期刊>Nature >Temperature-scan cryocrystallography reveals reaction intermediates in bacteriophytochrome
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Temperature-scan cryocrystallography reveals reaction intermediates in bacteriophytochrome

机译：温度扫描冷冻晶体分析揭示了细菌植物色素中的反应中间体

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摘要

Light is a fundamental signal that regulates important physiological processes such as development and circadian rhythm in living organisms. Phytochromes form a major family of photore-ceptors responsible for red light perception in plants, fungi and bacteria. They undergo reversible photoconversion between red-absorbing (Pr) and far-red-absorbing (Pfr) states, thereby ultimately converting a light signal into a distinct biological signal that mediates subsequent cellular responses2. Several structures of micro-bial phytochromes have been determined in their dark-adapted Pr or Pfr states. However, the structural nature of initial photochemical events has not been characterized by crystallography. Here we report the crystal structures of three intermediates in the photoreaction of Pseudomonas aeruginosa bacteriophytochrome (PaBphP). We used cryotrapping crystallography to capture intermediates, and followed structural changes by scanning the temperature at which the photo-reaction proceeded. Light-induced conformational changes in PaBphP originate in ring D of the biliverdin (BV) chromophore, and E-to-Z isomerization about the C_(15)=C_(16) double bond between rings C and D is the initial photochemical event. As the chromophore relaxes, the twist of the C_(15) methine bridge about its two dihedral angles is reversed. Structural changes extend further to rings B and A, and to the surrounding protein regions. These data indicate that absorption of a photon by the Pfr state of PaBphP converts a light signal into a structural signal via twisting and untwisting of the methine bridges in the linear tetrapyrrole within the confined protein cavity.%光敏色素是光受体，通过在红色和远红外光吸收状态之间的可逆光转换来调控植物、真菌和细菌的光反应。Yatlg等人利用温度扫描低温晶体学方法，获得了来自绿脓杆菌的一种细菌光敏色素在光反应期间出现的三个中间体和关键构形变化的结构信息。
机译：光是调节重要生理过程（例如生物体发育和昼夜节律）的基本信号。植物色素是光感受器的主要家族，负责植物，真菌和细菌中的红光感知。它们在吸收红光（Pr）和吸收远红光（Pfr）之间进行可逆的光转换，从而最终将光信号转换为介导后续细胞反应的独特生物信号2。已经确定了在其暗适应的Pr或Pfr状态下，微生物植物色素的几种结构。但是，初始光化学事件的结构性质尚未通过晶体学表征。在这里，我们报告铜绿假单胞菌细菌植物色素（PaBphP）的光反应中的三个中间体的晶体结构。我们使用了低温捕获晶体学来捕获中间体，并通过扫描光反应进行的温度来跟踪结构变化。 PaBphP的光诱导构象变化起源于Biliverdin（BV）生色团的D环，并且C和D环之间C_（15）= C_（16）双键的E-Z异构化是最初的光化学事件。随着生色团的松弛，C_（15）次甲基桥绕其两个二面角的扭曲被反转。结构变化进一步延伸到环B和A，以及周围的蛋白质区域。这些数据表明，PaBphP的Pfr状态吸收光子后，通过在受限蛋白腔内线性四吡咯中的次甲基桥扭曲和解旋，将光信号转换为结构信号。 Yatlg等人利用温度扫描低温晶体学方法，获得了来自绿脓杆菌的一种细菌光敏色素在光反应期间出现的三个中间和关键构形变化的结构信息。

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  • 来源
    《Nature》 |2011年第7373期|p.428-432|共5页
  • 作者

    Xiaojing Yang; Zhong Ren; Jane Kuk; Keith Moffat;

  • 作者单位

    Department of Biochemistry and Molecular Biology, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA;

    Centerfor Advanced Radiation Sources, The University of Chicago, 5610 South Ellis Avenue, Chicago, Illinois 60637, USA;

    Department of Biochemistry and Molecular Biology, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA;

    Department of Biochemistry and Molecular Biology, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA,Centerfor Advanced Radiation Sources, The University of Chicago, 5610 South Ellis Avenue, Chicago, Illinois 60637, USA,Institute for Biophysical Dynamics, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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