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Structural basis for the photoconversion of a phytochrome to the activated Pfr form

机译：将植物色素光转化为活化的Pfr形式的结构基础

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摘要

Phytochromes are a collection of bilin-containing photoreceptors that regulate numerous photoresponses in plants and microorganisms through their ability to photointerconvert between a red-light-absorbing, ground state (Pr) and a far-red-light-absorbing, photoactivated state (Pfr). Although the structures of several phytochromes as Pr have been determined, little is known about the structure of Pfr and how it initiates signalling. Here we describe the three-dimensional solution structure of the bilin-binding domain as Pfr, using the cyanobacterial phytochrome from Synechococcus OSB'. Contrary to predictions, light-induced rotation of the A pyrrole ring but not the D ring is the primary motion of the chromophore during photoconversion. Subsequent rearrangements within the protein then affect intradomain and interdomain contact sites within the phytochrome dimer. On the basis of our models, we propose that phytochromes act by propagating reversible light-driven conformational changes in the bilin to altered contacts between the adjacent output domains, which in most phytochromes direct differential phosphotransfer.
机译：植物色素是一组含胆碱的感光细胞，通过其在吸收红光的基态（Pr）和吸收远红光的光活化态（Pfr）之间进行光互转换的能力，调节植物和微生物中的多种光响应。 。尽管已经确定了几种植物色素的结构，如Pr，但对Pfr的结构及其如何引发信号的了解却很少。在这里，我们使用来自Synechococcus OSB'的蓝细菌植物色素，将Bilin结合域的三维溶液结构描述为Pfr。与预测相反，光转化过程中生色团的主要运动是A吡咯环而不是D环的光诱导旋转。蛋白质内的后续重排随后影响植物色素二聚体内的域内和域间接触位点。在我们的模型的基础上，我们提出植物色素的作用是通过传播胆汁中可逆的光驱动构象变化，以改变相邻输出域之间的接触，这在大多数植物色素中都可指导差异磷酸转移。

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  • 来源
    《Nature》 |2010年第7278期|250-254|共5页
  • 作者

    Andrew T. Ulijasz; Gabriel Cornilescu; Claudia C. Cornilescu; Junrui Zhang; Mario Rivera; John L. Markley; Richard D. Vierstra;

  • 作者单位

    Department of Genetics and National Magnetic Resonance Facility at Madison, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA;

    National Magnetic Resonance Facility at Madison, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA;

    National Magnetic Resonance Facility at Madison, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA;

    Department of Genetics and National Magnetic Resonance Facility at Madison, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA;

    Multidisciplinary Research Building, University of Kansas, Lawrence, Kansas 66057-1620, USA;

    National Magnetic Resonance Facility at Madison, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA;

    Department of Genetics and National Magnetic Resonance Facility at Madison, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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