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A synchronized quorum of genetic clocks

机译：基因时钟的同步定额

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摘要

The engineering of genetic circuits with predictive functionality in living cells represents a defining focus of the expanding field of synthetic biology. This focus was elegantly set in motion a decade ago with the design and construction of a genetic toggle switch and an oscillator, with subsequent highlights that have included circuits capable of pattern generation, noise shaping, edge detection and event counting. Here we describe an engineered gene network with global intercellular coupling that is capable of generating synchronized oscillations in a growing population of cells. Using microfluidic devices tailored for cellular populations at differing length scales, we investigate the collective synchronization properties along with spatiotemporal waves occurring at millimetre scales. We use computational modelling to describe quantitatively the observed dependence of the period and amplitude of the bulk oscillations on the flow rate. The synchronized genetic clock sets the stage for the use of microbes in the creation of a macroscopic biosensor with an oscillatory output. Furthermore, it provides a specific model system for the generation of a mechanistic description of emergent coordinated behaviour at the colony level.
机译：在活细胞中具有预测功能的遗传电路工程代表了合成生物学不断扩展的领域的确定焦点。十年前，通过设计和构造遗传拨动开关和振荡器，就很好地激发了这一关注点，随后的亮点包括能够进行码型生成，噪声整形，边缘检测和事件计数的电路。在这里，我们描述了具有全局细胞间偶联的工程基因网络，该网络能够在不断增长的细胞群体中产生同步振荡。使用针对不同长度尺度上的细胞群体量身定制的微流控设备，我们研究了集体同步特性以及在毫米尺度上发生的时空波。我们使用计算模型来定量描述所观察到的体积振荡的周期和幅度对流速的依赖性。同步遗传时钟为创建具有振荡输出的宏观生物传感器奠定了微生物使用的舞台。此外，它提供了一个特定的模型系统，用于生成殖民地一级紧急协调行为的机械描述。

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  • 来源
    《Nature》 |2010年第7279期|326-330|共5页
  • 作者

    Tal Danino; Octavio Mondragon-Palomino; Lev Tsimring; Jeff Hasty;

  • 作者单位

    Department of Bioengineering, University of California, San Diego, La Jolla, California 92093, USA;

    Department of Bioengineering, University of California, San Diego, La Jolla, California 92093, USA;

    BioCircuits Institute, University of California, San Diego, La Jolla, California 92093, USA;

    Department of Bioengineering, University of California, San Diego, La Jolla, California 92093, USA BioCircuits Institute, University of California, San Diego, La Jolla, California 92093, USA Molecular Biology Section Division of Biological Science University of California, Mailcode 0368, La Jolla, California 92093, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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