Taking a long, hard look

Monya Baker

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Taking a long, hard look

机译：仔细看一眼

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In the late 1980s, Sally Temple was studying neural development in mice at the University of Miami in Florida and needed a way to observe neural progenitor cells for days on end. At the time, no one had observed mammalian cells for more than a few hours, because the conditions that could be maintained under a microscope were too dry, cold and oxygen-rich to keep cells alive for long. Undeterred by the lack of precedent, Temple decided to build her own device that could monitor cells around the clock.rnTemple, a developmental neuroscientist now at the Neural Stem Cell Institute in Rensselaer, New York, credits her husband Jeffrey Stern with the inspiration for her apparatus. "He said, 'if the cells are living well in the incubator, you have to put the microscope in the incubator'," she recalls.

机译：在1980年代后期，萨利·邓普（Sally Temple）在佛罗里达州迈阿密大学研究小鼠的神经发育，并需要一种连续几天观察神经祖细胞的方法。当时，没有人能观察到哺乳动物细胞超过几个小时，因为在显微镜下可以维持的条件太干燥，太冷且富含氧气，无法使细胞长期存活。坦普尔（Temple）不受制于缺乏先例，因此决定建立自己的设备来全天候监控细胞。仪器。她回忆说：“他说，'如果细胞在培养箱中生活良好，则必须将显微镜放入培养箱中。”

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《Nature》 |2010年第7310期|P.1137-1140|共4页
作者
Monya Baker;
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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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Taking a long, hard look

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