Beyond fluorescence

BRUCE E.COHEN

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Beyond fluorescence

机译：超越荧光

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Biologists love optical microscopy. Open any cell-biology journal and you will be struck by a collection of colourful microscopy images worthy of a Seurat retrospective, with fluorescent staining patterns that help to unravel the complexities of cell signalling and development, neuroscience and cancer. But while microscopes have grown steadily more sophisticated over the years, so much so that single-molecule bioimaging is no longer uncommon, many of the fluorescent probes used for microscopy have remained unchanged for decades or longer.

机译：生物学家喜欢光学显微镜。打开任何细胞生物学期刊，您将被一系列值得Seurat回顾的彩色显微镜图像所吸引，这些图像具有荧光染色模式，有助于揭示细胞信号与发育，神经科学和癌症的复杂性。但是，尽管这些年来，显微镜已经变得越来越复杂，以至于单分子生物成像不再罕见，但用于显微镜的许多荧光探针却保持了数十年甚至更长的时间。

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来源
《Nature》 |2010年第7314期|P.407-408|共2页
作者
BRUCE E.COHEN;
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作者单位

Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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机译：基于Aptamer Nanoswitch触发的金属增强荧光的“荧光”从“荧光”中的ATP检测到“增强荧光”
2. Processing Excitation-Emission Matrix Fluorescence and Total Synchronous Fluorescence Spectroscopy Data Sets with Constraint Randomised Non-negative Factor Analysis: a Novel Fluorescence Based Analytical Procedure to Analyse the Multifluorophoric Mixtures [J] . Kumar Keshav Journal of Fluorescence . 2018,第5期

机译：加工激发发射矩阵荧光和总同步荧光光谱数据集，具有约束随机性非负因子分析：基于新的荧光分析方法分析多氟化物混合物
3. An immunofluorescence assay for extracellular matrix components highlights the role of epithelial cells in producing a stable, fibrillar extracellular matrix An immunofluorescence assay for extracellular matrix components highlights the role of epithelial cells in producing a stable, fibrillar extracellular matrix An immunofluorescence assay for extracellular matrix components highlights the role of epithelial cells in producing a stable, fibrillar extracellular matrix [J] . Kirsty Ford, Marianne Bergin, Omar S. Qureshi, Biology Open . 2017,第10期

机译：细胞外基质成分的免疫荧光分析突出了上皮细胞在产生稳定的，纤维状细胞外基质中的作用。细胞外基质成分的免疫荧光分析突出了上皮细胞在产生稳定的，纤维状细胞外基质中的作用。上皮细胞在产生稳定的纤维状细胞外基质中的作用
4. Fluorescence Recovery After Photobleaching (FRAP) with simultaneous Fluorescence Lifetime and time-resolved Fluorescence Anisotropy Imaging (FLIM tr-FAIM) [C] . Y. Teijeiro-Gonzalez, A. Le Marois, A.M. Economou, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI . 2019

机译：光漂白后的荧光恢复（FRAP），同时具有荧光寿命和时间分辨的荧光各向异性成像（FLIM＆tr-FAIM）
5. Development of High-Throughput Fluorescence Polarization Immunoassay System by a Fluorescence Polarization Imaging Technique [D] . 若尾, 摂 2019

机译：荧光偏振成像技术开发高通量荧光偏振免疫分析系统
6. Determination of the Residual Anthracene Concentration in Cultures of Haloalkalitolerant Actinomycetes by Excitation Fluorescence Emission Fluorescence and Synchronous Fluorescence: Comparative Study [O] . Reyna del Carmen Lara-Severino, Miguel Ángel Camacho-López, Jessica Marlene García-Macedo, 2016

机译：激发荧光发射荧光和同步荧光测定卤代链烷醇放线菌培养物中蒽的残留浓度：比较研究
7. Front-face fluorescence spectroscopy of tryptophan and fluorescein using laser induced fluorescence and excitation emission matrix fluorescence [O] . Stéphane Mounier, Roland Redon, Gustavo Nicolodelli, 2017

机译：色氨酸和荧光荧光素的前面荧光光谱法使用激光诱导荧光和激发发射矩阵荧光

Beyond fluorescence

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