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Membrane Scission By The Escrt-Ⅲ Complex

机译：Escrt-Ⅲ复合物的膜分裂

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摘要

The endosomal sorting complex required for transport (ESCRT) system is essential for multivesicular body biogenesis, in which cargo sorting is coupled to the invagination and scission of intralumenal vesicles. The ESCRTs are also needed for budding of enveloped viruses including human immunodeficiency virus 1, and for membrane abscission in cytokinesis. In Saccharomyces cerevisiae, ESCRT-Ⅲ consists of Vps20, Snf7, Vps24 and Vps2 (also known as Did4), which assemble in that order and require the ATPase Vps4 for their disassembly. In this study, the ESCRT-Ⅲ-dependent budding and scission of intralumenal vesicles into giant unilamellar vesicles was reconstituted and visualized by fluorescence microscopy. Here we show that three subunits of ESCRT-Ⅲ, Vps20, Snf7 and Vps24, are sufficient to detach intralumenal vesicles. Vps2, the ESCRT-Ⅲ subunit responsible for recruiting Vps4, and the ATPase activity of Vps4 were required for ESCRT-Ⅲ recycling and supported additional rounds of budding. The minimum set of ESCRT-Ⅲ and Vps4 proteins capable of multiple cycles of vesicle detachment corresponds to the ancient set of ESCRT proteins conserved from archaea to animals.
机译：运输所需的内体分选复合体（ESCRT）对于多囊体生物发生至关重要，其中货物分选与腔内囊泡的内陷和分裂相关。 ESCRT也需要包膜病毒（包括人免疫缺陷病毒1）出芽以及胞质分裂中的膜脱落。在酿酒酵母中，ESCRT-Ⅲ由Vps20，Snf7，Vps24和Vps2（也称为Did4）组成，它们以该顺序组装并需要ATPase Vps4才能拆卸。在这项研究中，ESCRT-Ⅲ依赖的出芽和切断腔内囊泡成巨大的单层囊泡，并通过荧光显微镜观察。在这里，我们显示ESCRT-Ⅲ的三个亚基，Vps20，Snf7和Vps24，足以分离腔内囊泡。 Vps2是负责招募Vps4的ESCRT-Ⅲ亚基，而Vps4的ATPase活性是ESCRT-Ⅲ回收所必需的，并支持新一轮的出芽。能够进行多次囊泡脱离的ESCRT-Ⅲ和Vps4蛋白的最小集合对应于从古细菌到动物保守的古代ESCRT蛋白集合。

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  • 来源
    《Nature》 |2009年第7235期|p.172-177|共6页
  • 作者

    Thomas Wollert; Christian Wunder; Jennifer Lippincott-Schwartz; James H. Hurley;

  • 作者单位

    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, US Department of Health and Human Services, Bethesda, Maryland 20892, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 自然科学总论;
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