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首页> 外文期刊>Nature >Ubiquitin-related Modifier Urm1 Acts As A Sulphur Carrier In Thiolation Of Eukaryotic Transfer Rna
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Ubiquitin-related Modifier Urm1 Acts As A Sulphur Carrier In Thiolation Of Eukaryotic Transfer Rna

机译：泛素相关修饰剂Urm1在真核转移RNA硫醇化中充当硫载体。

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摘要

Ubiquitin-like proteins (UBLs) can change protein function, localization or turnover by covalent attachment to lysine residues. Although UBLs achieve this conjugation through an intricate enzymatic cascade, their bacterial counterparts MoaD and ThiS function as sulphur carrier proteins. Here we show that Urmlp, the most ancient UBL, acts as a sulphur carrier in the process of eukaryotic transfer RNA (tRNA) modification, providing a possible evolutionary link between UBL and sulphur transfer. Moreover, we identify Uba4p, Ncs2p, Ncs6p and Yor251cp as components of this conserved pathway. Using in vitro assays, we show that Ncs6p binds to tRNA, whereas Uba4p first adenylates and then directly transfers sulphur onto Urmlp. Finally, functional analysis reveals that the thiolation function of Urmlp is critical to regulate cellular responses to nutrient starvation and oxidative stress conditions, most likely by-increasing translation fidelity.
机译：泛素样蛋白（UBL）可以通过共价附于赖氨酸残基来改变蛋白功能，定位或周转率。尽管UBL通过复杂的酶促级联反应实现了这种偶联，但它们的细菌对应物MoaD和ThiS却充当了硫载体蛋白。在这里，我们显示了最古老的UBL Urmlp在真核转移RNA（tRNA）修饰过程中充当硫载体，提供了UBL和硫转移之间可能的进化联系。此外，我们确定Uba4p，Ncs2p，Ncs6p和Yor251cp作为此保守途径的组成部分。使用体外试验，我们显示Ncs6p结合到tRNA，而Uba4p首先是腺苷酸，然后直接将硫转移到Urmlp上。最后，功能分析表明，Urmlp的硫醇化功能对于调节细胞对营养饥饿和氧化应激条件的反应至关重要，最有可能通过提高翻译保真度来实现。

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