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Self-assembly of a nanoscale DNA box with a controllable lid

机译:具有可控制盖的纳米级DNA盒的自组装

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摘要

The unique structural motifs and self-recognition properties of DNA can be exploited to generate self-assembling DNA nano-structures of specific shapes using a 'bottom-up' approach. Several assembly strategies have been developed for building complex three-dimensional (3D) DNA nanostructures. Recently, the DNA 'origami' method was used to build two-dimensional addressable DNA structures of arbitrary shape that can be used as platforms to arrange nanomaterials with high precision and specificity. A long-term goal of this field has been to construct fully addressable 3D DNA nanostructures. Here we extend the DNA origami method into three dimensions by creating an addressable DNA box 42 × 36 × 36 nm~3 in size that can be opened in the presence of externally supplied DNA 'keys'. We thoroughly characterize the structure of this DNA box using cryogenic transmission electron microscopy, small-angle X-ray scattering and atomic force microscopy, and use fluorescence resonance energy transfer to optically monitor the opening of the lid. Controlled access to the interior compartment of this DNA nanocontainer could yield several interesting applications, for example as a logic sensor for multiple-sequence signals or for the controlled release of nanocargos.
机译:可以利用“自下而上”的方法来利用DNA的独特结构基序和自我识别特性来生成特定形状的自组装DNA纳米结构。已经开发了几种组装策略来构建复杂的三维(3D)DNA纳米结构。最近,DNA的“折纸”方法被用于构建任意形状的二维可寻址DNA结构,可以用作平台以高精度和高特异性排列纳米材料。该领域的长期目标是构建完全可寻址的3D DNA纳米结构。在这里,我们通过创建一个可寻址的DNA盒(尺寸为42×36×36 nm〜3)将DNA折纸方法扩展到三个维度,该盒子可以在外部提供的DNA“钥匙”存在的情况下打开。我们使用低温透射电子显微镜,小角度X射线散射和原子力显微镜来彻底表征该DNA盒的结构,并使用荧光共振能量转移来光学监控盖子的打开。对该DNA纳米容器内部隔室的控制访问可能会产生一些有趣的应用,例如,作为多序列信号的逻辑传感器或纳米货物的受控释放的逻辑传感器。

著录项

  • 来源
    《Nature》 |2009年第7243期|73-76|共4页
  • 作者单位

    Danish National Research Foundation: Centre for DNA Nanotechnology, Aarhus University, DK-8000 Aarhus, Denmark Interdisciplinary Nanoscience Center, Aarhus University, DK-8000 Aarhus, Denmark Department of Molecular Biology, Aarhus University, DK-8000 Aarhus, Denmark;

    Danish National Research Foundation: Centre for DNA Nanotechnology, Aarhus University, DK-8000 Aarhus, Denmark Interdisciplinary Nanoscience Center, Aarhus University, DK-8000 Aarhus, Denmark Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus, Denmark Rowland Institute at Harvard, Harvard University, 100 Edwin H. Land Boulevard, Cambridge, Massachusetts 02142, USA;

    Danish National Research Foundation: Centre for DNA Nanotechnology, Aarhus University, DK-8000 Aarhus, Denmark Interdisciplinary Nanoscience Center, Aarhus University, DK-8000 Aarhus, Denmark Department of Molecular Biology, Aarhus University, DK-8000 Aarhus, Denmark;

    Danish National Research Foundation: Centre for DNA Nanotechnology, Aarhus University, DK-8000 Aarhus, Denmark Interdisciplinary Nanoscience Center, Aarhus University, DK-8000 Aarhus, Denmark Department of Molecular Biology, Aarhus University, DK-8000 Aarhus, Denmark;

    Danish National Research Foundation: Centre for DNA Nanotechnology, Aarhus University, DK-8000 Aarhus, Denmark Interdisciplinary Nanoscience Center, Aarhus University, DK-8000 Aarhus, Denmark Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus, Denmark;

    Danish National Research Foundation: Centre for DNA Nanotechnology, Aarhus University, DK-8000 Aarhus, Denmark Interdisciplinary Nanoscience Center, Aarhus University, DK-8000 Aarhus, Denmark Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus, Denmark;

    The Water and Salt Research Center, Institute of Anatomy, Aarhus University, DK-8000 Aarhus, Denmark Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D-37077 Gottingen, Germany;

    Stereology and EM Research Laboratory, Aarhus University, DK-8000 Aarhus, Denmark Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D-37077 Gottingen, Germany;

    Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D-37077 Gottingen, Germany Goettingen Centre for Molecular Biology, Justus-von-Liebig-Weg 11, University of Goettingen, D-37077 Goettingen, Germany;

    Interdisciplinary Nanoscience Center, Aarhus University, DK-8000 Aarhus, Denmark Department of Chemistry, Aarhus University, DK-8000 Aarhus, Denmark;

    Interdisciplinary Nanoscience Center, Aarhus University, DK-8000 Aarhus, Denmark Department of Chemistry, Aarhus University, DK-8000 Aarhus, Denmark;

    Interdisciplinary Nanoscience Center, Aarhus University, DK-8000 Aarhus, Denmark;

    Danish National Research Foundation: Centre for DNA Nanotechnology, Aarhus University, DK-8000 Aarhus, Denmark Interdisciplinary Nanoscience Center, Aarhus University, DK-8000 Aarhus, Denmark Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus, Denmark;

    Danish National Research Foundation: Centre for DNA Nanotechnology, Aarhus University, DK-8000 Aarhus, Denmark Interdisciplinary Nanoscience Center, Aarhus University, DK-8000 Aarhus, Denmark Department of Chemistry, Aarhus University, DK-8000 Aarhus, Denmark;

    Danish National Research Foundation: Centre for DNA Nanotechnology, Aarhus University, DK-8000 Aarhus, Denmark Interdisciplinary Nanoscience Center, Aarhus University, DK-8000 Aarhus, Denmark Department of Molecular Biology, Aarhus University, DK-8000 Aarhus, Denmark;

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