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Structures of the tRNA export factor in the nuclear and cytosolic states

机译：处于核和胞质状态的tRNA出口因子的结构

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摘要

Transfer RNAs are among the most ubiquitous molecules in cells, central to decoding information from messenger RNAs on translating ribosomes. In eukaryotic cells, tRNAs are actively transported from their site of synthesis in the nucleus to their site of function in the cytosol. This is mediated by a dedicated nucleo-cytoplasmic transport factor of the karyopherin-β family (Xpot, also known as Los1 in Saccharomyces cerevisiae). Here we report the 3.2 A resolution structure of Schizosaccharomyces pombe Xpot in complex with tRNA and RanGTP, and the 3.1 A structure of unbound Xpot, revealing both nuclear and cytosolic snapshots of this transport factor. Xpot undergoes a large conformational change on binding cargo, wrapping around the tRNA and, in particular, binding to the tRNA 5' and 3' ends. The binding mode explains how Xpot can recognize all mature tRNAs in the cell and yet distinguish them from those that have not been properly processed, thus coupling tRNA export to quality control.
机译：转移RNA是细胞中最普遍存在的分子之一，在翻译核糖体时从信使RNA解码信息至关重要。在真核细胞中，tRNA从其在细胞核中的合成位点主动转运到其在细胞质中的功能位点。这是由karyopherin-β家族的专用核细胞质转运因子介导的（Xpot，在酿酒酵母中也称为Los1）。在这里，我们报告了与tRNA和RanGTP结合的3.2粟酒裂殖酵母Xpot的解析结构，以及3.1 Unbound Xpot的结构，揭示了该转运因子的核和胞质快照。 Xpot在结合货物上经历了很大的构象变化，包裹在tRNA周围，尤其是与tRNA 5'和3'末端结合。结合模式解释了Xpot如何识别细胞中所有成熟的tRNA，并将它们与未正确处理的tRNA区别开，从而将tRNA的输出与质量控制相结合。

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  • 来源
    《Nature》 |2009年第7260期|60-65|共6页
  • 作者

    Atlanta G. Cook; Noemi Fukuhara; Martin Jinek; Elena Conti;

  • 作者单位

    Structural Cell Biology, MPI for Biochemistry, Am Klopferspitz 18,82152 Martinsried, Germany;

    EMBL, Meyerhofstrasse 1, Heidelberg D69117, Germany Division of Cell and Molecular Biology, Biophysics section, Blackett Laboratory, Imperial College London, London SW7 2AZ, UK (N.F ) Department of Molecular Cell Biology, University of California at Berkeley, 731 Stanley Hall, Berkeley, California 94720, USA;

    EMBL, Meyerhofstrasse 1, Heidelberg D69117, Germany;

    Structural Cell Biology, MPI for Biochemistry, Am Klopferspitz 18,82152 Martinsried, Germany EMBL, Meyerhofstrasse 1, Heidelberg D69117, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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