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iPS cells produce viable mice through tetraploid complementation

机译：iPS细胞通过四倍体互补产生活小鼠

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摘要

Since the initial description of induced pluripotent stem (iPS) cells created by forced expression of four transcription factors in mouse fibroblasts, the technique has been used to generate embryonic stem (ES)-cell-like pluripotent cells from a variety of cell types in other species, including primates and rat. It has become a popular means to reprogram somatic genomes into an embryonic-like pluripotent state, and a preferred alternative to somatic-cell nuclear transfer and somatic-cell fusion with ES cells. However, iPS cell reprogramming remains slow and inefficient. Notably, no live animals have been produced by the most stringent tetraploid complementation assay, indicative of a failure to create fully pluripotent cells. Here we report the generation of several iPS cell lines that are capable of generating viable, fertile live-born progeny by tetraploid complementation. These iPS cells maintain a pluripotent potential that is very close to ES cells generated from in vivo or nuclear transfer embryos. We demonstrate the practicality of using iPS cells as useful tools for the characterization of cellular reprogramming and developmental potency, and confirm that iPS cells can attain true pluripotency that is similar to that of ES cells.
机译：由于最初描述了通过在小鼠成纤维细胞中强制表达四种转录因子而产生的诱导多能干（iPS）细胞，该技术已被用于从其他细胞中的多种细胞类型中产生类似胚胎干（ES）细胞的多能细胞。物种，包括灵长类动物和大鼠。它已成为将体细胞基因组重编程为类胚胎多能状态的一种流行手段，并且是体细胞核移植和与ES细胞融合的首选方法。但是，iPS细胞重编程仍然缓慢且效率低下。值得注意的是，通过最严格的四倍体互补测定没有产生活体动物，这表明不能产生完全多能的细胞。在这里，我们报告了几种iPS细胞系的产生，这些细胞系能够通过四倍体互补产生有活力的，可育的活体后代。这些iPS细胞保持了多能性，非常接近体内或核移植胚胎产生的ES细胞。我们证明了使用iPS细胞作为表征细胞重编程和发育潜能的有用工具的实用性，并证实iPS细胞可以实现与ES细胞相似的真正多能性。

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  • 来源
    《Nature》 |2009年第7260期|86-90|共5页
  • 作者

    Xiao-yang Zhao; Wei Li; Zhuo Lv; Lei Liu; Man Tong; Tang Hai; Jie Hao; Chang-long Guo; Qing-wen Ma; Liu Wang; Fanyi Zeng; Qi Zhou;

  • 作者单位

    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, ChinarnGraduate School of Chinese Academy of Sciences, Beijing 100049, China;

    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, ChinarnGraduate School of Chinese Academy of Sciences, Beijing 100049, China;

    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, ChinarnGraduate School of Chinese Academy of Sciences, Beijing 100049, China;

    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China;

    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, ChinarnGraduate School of Chinese Academy of Sciences, Beijing 100049, China;

    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China;

    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China Graduate School of Chinese Academy of Sciences, Beijing 100049, China;

    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China Graduate School of Chinese Academy of Sciences, Beijing 100049, China;

    Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai 200040, China;

    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China;

    Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai 200040, China Institute of Medical Science, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China;

    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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