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Structure of the outer membrane complex of a type IV secretion system

机译：IV型分泌系统外膜复合物的结构

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摘要

Type IV secretion systems are secretion nanomachines spanning the two membranes of Gram-negative bacteria. Three proteins, VirB7, VirB9 and VirB10, assemble into a 1.05 megadalton (MDa) core spanning the inner and outer membranes. This core consists of 14 copies of each of the proteins and forms two layers, the I and O layers, inserting in the inner and outer membrane, respectively. Here we present the crystal structure of a ～0.6 MDa outer-membrane complex containing the entire O layer. This structure is the largest determined for an outer-membrane channel and is unprecedented in being composed of three proteins. Unexpectedly, this structure identifies VirBIO as the outer-membrane channel with a unique hydrophobic double-helical transmembrane region. This structure establishes VirBIO as the only known protein crossing both membranes of Gram-negative bacteria. Comparison of the cryo-electron microscopy (cryo-EM) and crystailographic structures points to conformational changes regulating channel opening and closing.
机译：IV型分泌系统是跨越革兰氏阴性细菌两膜的分泌纳米机器。 VirB7，VirB9和VirB10这三种蛋白质组装成1.05兆达尔顿（MDa）核心，横跨内膜和外膜。该核心由每种蛋白质的14个拷贝组成，并分别形成两层，即I和O层，分别插入内膜和外膜中。在这里，我们介绍了一个包含整个O层的〜0.6 MDa外膜复合物的晶体结构。这种结构是外膜通道最大的结构，并且由三种蛋白质组成，这是前所未有的。出乎意料的是，这种结构将VirBIO识别为具有独特的疏水性双螺旋跨膜区的外膜通道。这种结构使VirBIO成为唯一已知的跨革兰氏阴性细菌两膜的蛋白质。冷冻电子显微镜（cryo-EM）和冰晶仪结构的比较表明，构象变化调节通道的打开和关闭。

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  • 来源
    《Nature》 |2009年第7276期|1011-1015|共5页
  • 作者

    Vidya Chandran; Remi Fronzes; Stephane Duquerroy; Nora Cronin; Jorge Navaza; Gabriel Waksman;

  • 作者单位

    Institute of Structural and Molecular Biology, University College London and Birkbeck College, Malet Street, London WC1E 7HX, UK;

    Institute of Structural and Molecular Biology, University College London and Birkbeck College, Malet Street, London WC1E 7HX, UK;

    Institut Pasteur, Unite de ViroLogie Structurale Virology Department and CNRS URA 3015, Paris, 25-28 Rue du Dr Roux, F-75724 Paris, France Universite Paris-Sud, F-91105 Orsay, France;

    Institute of Structural and Molecular Biology, University College London and Birkbeck College, Malet Street, London WC1E 7HX, UK;

    Laboratoire de Microscople Electronique, Institut de Biologie Structural J.P. Ebel, 41 rue Jules Horowitz, F-38027 Grenoble Cedex1, France;

    Institute of Structural and Molecular Biology, University College London and Birkbeck College, Malet Street, London WC1E 7HX, UK;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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