• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nature >Charge- and size-based separation of macromolecules using ultrathin silicon membranes.
【24h】

Charge- and size-based separation of macromolecules using ultrathin silicon membranes.

机译：使用超薄硅膜基于电荷和大小的大分子分离。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Commercial ultrafiltration and dialysis membranes have broad pore size distributions and are over 1,000 times thicker than the molecules they are designed to separate, leading to poor size cut-off properties, filtrate loss within the membranes, and low transport rates. Nanofabricated membranes have great potential in molecular separation applications by offering more precise structural control, yet transport is also limited by micrometre-scale thicknesses. This limitation can be addressed by a new class of ultrathin nanostructured membranes where the membrane is roughly as thick (approximately 10 nm) as the molecules being separated, but membrane fragility and complex fabrication have prevented the use of ultrathin membranes for molecular separations. Here we report the development of an ultrathin porous nanocrystalline silicon (pnc-Si) membrane using straightforward silicon fabrication techniques that provide control over average pore sizes from approximately 5 nm to 25 nm. Our pnc-Si membranes can retain proteins while permitting the transport of small molecules at rates an order of magnitude faster than existing materials, separate differently sized proteins under physiological conditions, and separate similarly sized molecules carrying different charges. Despite being only 15 nm thick, pnc-Si membranes that are free-standing over 40,000 microm2 can support a full atmosphere of differential pressure without plastic deformation or fracture. By providing efficient, low-loss macromolecule separations, pnc-Si membranes are expected to enable a variety of new devices, including membrane-based chromatography systems and both analytical and preparative microfluidic systems that require highly efficient separations.
机译：商业超滤和渗析膜的孔径分布较宽，比其设计分离的分子厚1,000倍以上，从而导致尺寸截留性能差，膜内滤液损失少以及运输速率低。通过提供更精确的结构控制，纳米制造的膜在分子分离应用中具有巨大的潜力，但是运输也受到微米级厚度的限制。这种局限性可以通过一类新型的超薄纳米结构膜来解决，该膜的厚度与被分离的分子的厚度大致相同（约10 nm），但是膜的脆性和复杂的制备阻止了将超薄膜用于分子分离。在这里，我们报告了使用简单的硅制造技术开发的超薄多孔纳米晶体硅（pnc-Si）膜，该技术可控制从大约5 nm到25 nm的平均孔径。我们的pnc-Si膜可以保留蛋白质，同时允许小分子以比现有材料快一个数量级的速率运输，在生理条件下分离大小不同的蛋白质，以及分离大小不同的带有不同电荷的分子。尽管只有15 nm厚，可独立放置超过40,000 microm2的pnc-Si膜仍可支撑整个压差气氛，而不会发生塑性变形或破裂。通过提供高效，低损耗的大分子分离，PNC-Si膜有望实现多种新设备，包括基于膜的色谱系统以及需要高效分离的分析和制备型微流体系统。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号