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首页> 外文期刊>Nature >An unconventional myosin in Drosophila reverses the default handedness in visceral organs
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An unconventional myosin in Drosophila reverses the default handedness in visceral organs

机译：果蝇中非常规的肌球蛋白逆转内脏器官的默认惯用性

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The internal organs of animals often have left-right asymmetry. Although the formation of the anterior-posterior and dorsal-ventral axes in Drosophila is well understood, left-right asymmetry has not been extensively studied. Here we find that the handedness of the embryonic gut and the adult gut and testes is reversed (not randomized) in viable and fertile homozygous Myo31DF mutants. Myo31DF encodes an unconventional myosin, Drosophila MyoIA (also referred to as MyoID in mammals; refs 3, 4), and is the first actin-based motor protein to be implicated in left-right patterning. We find that Myo31DF is required in the hindgut epithelium for normal embryonic handedness. Disruption of actin filaments in the hindgut epithelium randomizes the handedness of the embryonic gut, suggesting that Myo31DF function requires the actin cytoskeleton. Consistent with this, we find that Myo31DF colocalizes with the cytoskeleton. Overexpression of Myo61F, another myosin Ⅰ (ref. 4), reverses the handedness of the embryonic gut, and its knockdown also causes a left-right patterning defect. These two unconventional myosin Ⅰ proteins may have antagonistic functions in left-right patterning. We suggest that the actin cytoskeleton and myosin Ⅰ proteins may be crucial for generating left-right asymmetry in invertebrates.
机译：动物的内脏通常具有左右不对称性。尽管果蝇中前后轴和背腹轴的形成已广为人知，但尚未广泛研究左右不对称性。在这里，我们发现，在活的和可育的纯合Myo31DF突变体中，胚胎肠道，成年肠道和睾丸的惯性被反转（不是随机的）。 Myo31DF编码一种非常规的肌球蛋白果蝇MyoIA（在哺乳动物中也称为MyoID；参考文献3、4），并且是第一个基于肌动蛋白的运动蛋白，涉及左右模式。我们发现，Myo31DF在后肠上皮中是正常胚胎惯用性所必需的。后肠上皮肌动蛋白丝的破坏使胚胎肠道的顺手性随机化，这表明Myo31DF功能需要肌动蛋白的细胞骨架。与此相一致，我们发现Myo31DF与细胞骨架共定位。 Myo61F（另一种肌球蛋白Ⅰ）的过表达（参考文献4）逆转了胚胎肠道的惯用性，其击倒也引起左右图案缺陷。这两种非常规的肌球蛋白Ⅰ蛋白在左右模式中可能具有拮抗作用。我们认为肌动蛋白的细胞骨架和肌球蛋白Ⅰ蛋白可能对于在无脊椎动物中产生左右不对称性至关重要。

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