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首页> 外文期刊>Nature >The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs
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The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs

机译：精子与卵融合需要免疫球蛋白超家族蛋白Izumo

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Representing the 60 trillion cells that build a human body, a sperm and an egg meet, recognize each other, and fuse to form a new generation of life. The factors involved in this important membrane fusion event, fertilization, have been sought for a long time(1). Recently, CD9 on the egg membrane was found to be essential for fusion(2-4), but sperm-related fusion factors remain unknown. Here, by using a fusion-inhibiting monoclonal antibody(5) and gene cloning, we identify a mouse sperm fusion-related antigen and show that the antigen is a novel immunoglobulin superfamily protein. We have termed the gene Izumo and produced a gene-disrupted mouse line. Izumo(-/-) mice were healthy but males were sterile. They produced normal-looking sperm that bound to and penetrated the zona pellucida but were incapable of fusing with eggs. Human sperm also contain Izumo and addition of the antibody against human Izumo left the sperm unable to fuse with zona-free hamster eggs.
机译：代表建立人体的60万亿个细胞，精子和卵子相遇，相互识别并融合形成新一代生命。长期以来一直在寻求与这种重要的膜融合事件有关的因素，即受精（1）。最近，发现蛋膜上的CD9是融合必不可少的（2-4），但与精子相关的融合因子仍然未知。在这里，通过使用融合抑制单克隆抗体（5）和基因克隆，我们确定了小鼠精子融合相关抗原，并表明该抗原是一种新型的免疫球蛋白超家族蛋白。我们称该基因为出云基因，并产生了基因破坏的小鼠品系。 Izumo（-/-）小鼠健康，但雄性不育。他们产生了看起来正常的精子，该精子结合并穿透了透明带，但无法与卵融合。人精子中还含有出云，抗人出云抗体的添加使精子无法与无透明带的仓鼠卵融合。

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