Crystal structure of a membrane-bound metalloenzyme that catalyses the biological oxidation of methane

Lieberman RL; Rosenzweig AC

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Crystal structure of a membrane-bound metalloenzyme that catalyses the biological oxidation of methane

机译：膜结合的金属酶的晶体结构，催化甲烷的生物氧化

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Particulate methane monooxygenase ( pMMO) is an integral membrane metalloenzyme that catalyses the conversion of methane to methanol. Knowledge of how pMMO performs this extremely challenging chemistry may have an impact on the use of methane as an alternative energy source by facilitating the development of new synthetic catalysts. We have determined the structure of pMMO from the methanotroph Methylococcus capsulatus ( Bath) to a resolution of 2.8 Angstrom. The enzyme is a trimer with an alpha(3)beta(3)gamma(3) polypeptide arrangement. Two metal centres, modelled as mononuclear copper and dinuclear copper, are located in soluble regions of each pmoB subunit, which resembles cytochrome c oxidase subunit II. A third metal centre, occupied by zinc in the crystal, is located within the membrane. The structure provides new insight into the molecular details of biological methane oxidation.

机译：颗粒甲烷单加氧酶（pMMO）是一种不可或缺的膜金属酶，可催化甲烷向甲醇的转化。关于pMMO如何执行这种极具挑战性的化学反应的知识可能会通过促进新型合成催化剂的开发，而对甲烷作为替代能源的使用产生影响。我们已经确定了从甲烷营养缺陷型甲基球菌（Bath）到2.8埃分辨率的pMMO的结构。该酶是具有alpha（3）beta（3）gamma（3）多肽排列的三聚体。建模为单核铜和双核铜的两个金属中心位于每个pmoB亚基的可溶区域，类似于细胞色素c氧化酶亚基II。膜中有第三个金属中心，被晶体中的锌占据。该结构为生物甲烷氧化的分子细节提供了新的见识。

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来源
《Nature》 |2005年第7030期|p. 177-182|共6页
作者
Lieberman RL; Rosenzweig AC;
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作者单位

Northwestern Univ, Dept Biochem Mol Biol & Cell Biol, Evanston, IL 60208 USA;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词
METHYLOCOCCUS-CAPSULATUS BATH; CYTOCHROME-C-OXIDASE; PARACOCCUS-DENITRIFICANS; METHANOTROPHIC BACTERIA; OXIDIZING BACTERIA; ELECTRON-TRANSFER; MONOOXYGENASE; PROTEINS; COPPER; ACTIVATION;

机译：甲烷球囊浴;细胞色素C-氧化酶;PARACOCCUS-DENITRIFICANS;嗜热菌;氧化菌;电子转移;单氧合酶;蛋白质;铜;活化;

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2. Axial Changes of Catalyst Structure and Temperature in a Fixed-Bed Microreactor During Noble Metal Catalysed Partial Oxidation of Methane [J] . Stefan Hannemann, Jan-Dierk Grunwaldt, Bertram Kimmerle Topics in Catalysis . 2009,第10期

机译：贵金属催化的甲烷部分氧化过程中固定床微反应器中催化剂结构和温度的轴向变化
3. Axial Changes of Catalyst Structure and Temperature in a Fixed-Bed Microreactor During Noble Metal Catalysed Partial Oxidation of Methane [J] . Stefan Hannemann, Jan-Dierk Grunwaldt, Bertram Kimmerle, Topics in Catalysis . 2009,第10期

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4. Comparison of nanocrystalline and polycrystalline oxide supports for catalytic oxidation of methane [C] . Horst H. Hahn, Heiko Hesemann, William S. Epling, Symposium on recent advances in catalytic materials . 1998

机译：纳米晶体和多晶氧化物溶液对甲烷催化氧化的比较
5. Biological methane oxidation: Biochemical, biophysical, and structural characterization of particulate methane monooxygenase from Methylococcus capsulatus (Bath) and Methylocystis sp. strain M. [D] . Smith, Stephen Michael. 2011

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Crystal structure of a membrane-bound metalloenzyme that catalyses the biological oxidation of methane

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