• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nature >The histone H3.3 chaperone HIRA is essential for chromatin assembly in the male pronucleus
【24h】

The histone H3.3 chaperone HIRA is essential for chromatin assembly in the male pronucleus

机译：组蛋白H3.3伴侣HIRA对于男性前核染色质组装至关重要

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In sexually reproducing animals, a crucial step in zygote formation is the decondensation of the fertilizing sperm nucleus into a DNA replication-competent male pronucleus. Genome-wide nucleosome assembly on paternal DNA implies the replacement of sperm chromosomal proteins, such as protamines, by maternally provided histones. This fundamental process is specifically impaired in sesame (ssm), a unique Drosophila maternal effect mutant that prevents male pronucleus formation. Here we show that ssm is a point mutation in the Hira gene, thus demonstrating that the histone chaperone protein HIRA is required for nucleosome assembly during sperm nucleus decondensation. In vertebrates, HIRA has recently been shown to be critical for a nucleosome assembly pathway independent of DNA synthesis that specifically involves the H3.3 histone variant. We also show that nucleosomes containing H3.3, and not H3, are specifically assembled in paternal Drosophila chromatin before the first round of DNA replication. The exclusive marking of paternal chromosomes with H3.3 represents a primary epigenetic distinction between parental genomes in the zygote, and underlines an important consequence of the critical and highly specialized function of HIRA at fertilization.
机译：在有性繁殖的动物中，合子形成的关键步骤是将受精精子核解聚为具有DNA复制能力的雄性原核。父本DNA上的全基因组核小体组装意味着由母体提供的组蛋白替代了精子染色体蛋白（如鱼精蛋白）。芝麻（ssm）是一种独特的果蝇母体效应突变体，可防止男性前核形成，因此特别损害了这一基本过程。在这里，我们显示ssm是Hira基因中的一个点突变，因此表明在精子核解聚过程中，组蛋白分子伴侣蛋白HIRA是核小体组装所必需的。在脊椎动物中，最近显示出HIRA对于独立于DNA合成的核小体组装途径至关重要，该途径特别涉及H3.3组蛋白变体。我们还表明，在第一轮DNA复制之前，含有H3.3而不是H3的核小体是在父本果蝇染色质中专门组装的。用H3.3对父本染色体进行的唯一标记代表合子中父母基因组之间的主要表观遗传学区别，并强调了HIRA在受精过程中的关键性和高度专门化功能的重要结果。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号