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Coupled oscillators control morning and evening locomotor behaviour of Drosophila

机译:耦合振荡器控制果蝇的早晚运动行为

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摘要

Daily rhythms of physiology and behaviour are precisely timed by an endogenous circadian clock(1,2). These include separate bouts of morning and evening activity, characteristic of Drosophila melanogaster and many other taxa, including mammals(3-5). Whereas multiple oscillators have long been proposed to orchestrate such complex behavioural programmes(6), their nature and interplay have remained elusive. By using cell-specific ablation, we show that the timing of morning and evening activity in Drosophila derives from two distinct groups of circadian neurons: morning activity from the ventral lateral neurons that express the neuropeptide PDF, and evening activity from another group of cells, including the dorsal lateral neurons. Although the two oscillators can function autonomously, cell-specific rescue experiments with circadian clock mutants indicate that they are functionally coupled.
机译:生理和行为的日常节律由内源性生物钟精确计时(1,2)。其中包括早晨和晚上活动的分开,果蝇和其他许多类群的特征,包括哺乳动物(3-5)。尽管长期以来一直建议使用多个振荡器来组织这种复杂的行为程序(6),但它们的性质和相互作用仍然难以捉摸。通过使用细胞特异性消融,我们显示了果蝇中早晨和傍晚活动的时间来自两个不同的昼夜节律神经元组:来自表达神经肽PDF的腹侧神经元的早晨活动,以及来自另一组细胞的夜间活动,包括背外侧神经元。尽管这两个振荡器可以自主运行,但使用生物钟时钟突变体的特定于细胞的抢救实验表明它们在功能上是耦合的。

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