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The plant MITE mPing is mobilized in anther culture

机译:植物MITE mPing在花药培养中动员

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Transposable elements constitute a large portion of eukaryotic genomes and contribute to their evolution and diversification. Miniature inverted-repeat transposable elements (MITEs) constitute one of the main groups of transposable elements and are distributed ubiquitously in the genomes of plants and animals such as maize, rice, Arabidopsis, human, insect and nematode. Because active MITEs have not been identified, the transposition mechanism of MITEs and their accumulation in eukaryotic genomes remain poorly understood. Here we describe a new class of MITE, called miniature Ping (mPing), in the genome of Oryza sativa (rice). mPing elements are activated in cells derived from anther culture, where they are excised efficiently from original sites and reinserted into new loci. An mPing-associated Ping element, which has a putative PIF family transposase, is implicated in the recent proliferation of this MITE family in a subspecies of rice.
机译:转座因子构成了真核生物基因组的很大一部分,并促进了它们的进化和多样化。微型反向重复转座因子(MITE)构成了转座因子的主要组之一,并普遍存在于动植物基因组中,例如玉米,水稻,拟南芥,人类,昆虫和线虫。由于尚未鉴定出活性的MITE,因此对MITE的转座机制及其在真核基因组中的积累仍然知之甚少。在这里,我们描述了水稻基因组中一类新型的MITE,称为微型Ping(mPing)。 mPing元件在源自花药培养的细胞中被激活,在那里它们被有效地从原始位点切除并重新插入新的基因座。 mPing相关的Ping元件具有推定的PIF家族转座酶,与该MITE家族最近在水稻亚种中的增殖有关。

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