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首页> 外文期刊>Nature >A self-organizing system of repressor gradients establishes segmental complexity in Drosophila
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A self-organizing system of repressor gradients establishes segmental complexity in Drosophila

机译：一个自组织的阻遏物梯度系统建立了果蝇的节段复杂性

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Gradients of regulatory factors are essential for establishing precise patterns of gene expression during development; however, it is not clear how patterning information in multiple gradients is integrated to generate complex body plans. Here we show that opposing gradients of two Drosophila transcriptional repressors, Hunchback (Hb) and Knirps (Kni), position several segments by differentially repressing two distinct regulatory regions (enhancers) of the pair-rule gene even-skipped (eve). Computational and in vivo analyses suggest that enhancer sensitivity to repression is controlled by the number and affinity of repressor-binding sites. Because the kni expression domain is positioned between two gradients of Hb, each enhancer directs expression of a pair of symmetrical stripes, one on each side of the kni domain. Thus, only two enhancers are required for the precise positioning of eight stripe borders (four stripes), or more than half of the whole eve pattern. Our results show that complex developmental expression patterns can be generated by simple repressor gradients. They also support the utility of computational analyses for defining and deciphering regulatory information contained in genomic DNA.
机译：调控因子的梯度对于在发育过程中建立精确的基因表达模式至关重要。但是，尚不清楚如何将多个渐变中的图案信息集成在一起以生成复杂的人体计划。在这里，我们显示了两个果蝇转录阻遏物，Hunchback（Hb）和Knirps（Kni）的相反梯度，通过差异抑制两个规则（偶数）对规则基因的两个不同调控区（增强子）来定位几个片段。计算和体内分析表明，增强子对阻遏物的敏感性由阻遏物结合位点的数量和亲和力控制。由于kni表达结构域位于Hb的两个梯度之间，因此每个增强子指导一对对称条带的表达，在kni结构域的每一侧都有一条。因此，仅需要两个增强器即可精确定位八个条纹边界（四个条纹），或整个前夕图案的一半以上。我们的结果表明，复杂的发育表达模式可以通过简单的阻遏物梯度产生。他们还支持使用计算分析来定义和破译基因组DNA中包含的调控信息。

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