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首页> 外文期刊>Nature >Role for a Drosophila Myb-containing protein complex in site-specific DNA replication
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Role for a Drosophila Myb-containing protein complex in site-specific DNA replication

机译：果蝇含Myb的蛋白质复合体在位点特异性DNA复制中的作用

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There is considerable interest in the developmental, temporal and tissue-specific patterns of DNA replication in metazoans. Site-specific DNA replication at the chorion loci in Drosophila follicle cells leads to extensive gene amplification, and the organization of the as-acting DNA elements that regulate this process may provide a model for how such regulation is achieved. Two elements important for amplification of the third chromosome chorion gene cluster, ACE3 and Ori-β, are directly bound by Orc (origin recognition complex), and two-dimensional gel analysis has revealed that the primary origin used is Ori-β (refs 7―9). Here we show that the Drosophila homologue of the Myb (Myeloblastosis) oncoprotein family is tightly associated with four additional proteins, and that the complex binds site-specifically to these regulatory DNA elements. Drosophila Myb is required in trans for gene amplification, showing that a Myb protein is directly involved in DNA replication. A Drosophila Myb binding site, as well as the binding site for another Myb complex member (p120), is necessary in cis for replication of reporter transgenes. Chromatin immuno-precipitation experiments localize both proteins to the chorion loci in vivo. These data provide evidence that specific protein complexes bound to replication enhancer elements work together with the general replication machinery for site-specific origin utilization during replication.
机译：在后生动物中，DNA复制的发育，时间和组织特异性模式引起了人们的极大兴趣。果蝇滤泡细胞在绒毛膜基因座上的位点特异性DNA复制导致广泛的基因扩增，调节该过程的作用DNA元件的组织可能为实现这种调节提供一个模型。 Orc（起源识别复合物）直接结合了对第三条染色体绒毛膜基因簇的扩增很重要的两个元素，即ACE3和Ori-β，并且二维凝胶分析表明所用的主要来源是Ori-β（参考文献7 ―9）。在这里，我们显示Myb（成纤维细胞增生）癌蛋白家族的果蝇同源物与四个其他蛋白紧密相关，并且该复合物位点特异性结合这些调节性DNA元件。果蝇Myb是反式基因扩增所必需的，这表明Myb蛋白直接参与DNA复制。果蝇Myb结合位点以及另一个Myb复杂成员（p120）的结合位点在顺式中对于报告基因转基因的复制是必需的。染色质免疫沉淀实验在体内将这两种蛋白质定位在绒毛膜基因座上。这些数据提供了证据，表明与复制增强子结合的特定蛋白质复合物可与通用复制机制协同工作，从而在复制过程中利用位点特异性来源。

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