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The DExH protein NPH-Ⅱ is a processive and directional motor for unwinding RNA

机译：DExH蛋白NPH-Ⅱ是一种用于解开RNA的过程性定向马达

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All aspects of cellular RNA metabolism and processing involve DExH/D proteins, which are a family of enzymes that unwind or manipulate RNA in an ATP-dependent fashion. DExH/D proteins are also essential for the replication of many viruses, and therefore provide targets for the development of therapeutics. All DExH/D proteins characterized to date hydrolyse nucleoside triphosphates and, in most cases, this activity is stimulated by the addition of RNA or DNA. Several members of the family unwind RNA duplexes in an NTP-dependent fashion in vitro; therefore it has been proposed that DExH/D proteins couple NTP hydrolysis to RNA conformational change in complex macromolecular assemblies. Despite the central role of DExH/D proteins, their mechanism of RNA helicase activity remains unknown. Here we show that the DExH protein NPH-Ⅱ unwinds RNA duplexes in a processive, unidirectional fashion with a step size of roughly one-half helix turn. We show that there is a quantitative connection between ATP utilization and helicase processivity, thereby providing direct evidence that DExH/D proteins can function as molecular motors on RNA.
机译：细胞RNA代谢和加工的所有方面都涉及DExH / D蛋白，它是一种以ATP依赖性方式解开或操纵RNA的酶家族。 DExH / D蛋白对于许多病毒的复制也是必不可少的，因此为治疗剂的开发提供了靶标。迄今为止，所有表征为DExH / D的蛋白都水解三磷酸核苷，并且在大多数情况下，添加RNA或DNA可以刺激这种活性。该家族的几个成员在体外以NTP依赖的方式解开RNA双链体。因此，有人提出DExH / D蛋白将NTP水解与复杂大分子装配体中的RNA构象变化耦合。尽管DExH / D蛋白起着核心作用，但其RNA解旋酶活性的机制仍然未知。在这里，我们显示DExH蛋白NPH-Ⅱ以逐步，单向的方式展开RNA双链体，步长约为半个螺旋圈。我们表明，ATP利用与解旋酶的合成能力之间存在定量联系，从而提供直接证据证明DExH / D蛋白可以充当RNA上的分子驱动器。

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