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Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA

机译：Let-7异时调节RNA的序列和时间表达的保守性

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摘要

Two small RNAs regulate the timing of Caenorhabditis elegans development. Transition from the first to the second larval stage fates requires the 22-nucleotide lin-4 RNA, and transition from late larval to adult cell fates requires the 21-nucleotide let-7 RNA. The lin-4 and let-7 RNA genes are not homologous to each other, but are each complementary to sequences in the 3' untranslated regions of a set of protein-coding target genes that are normally negatively regulated by the RNAs. Here we have detected let-7 RNAs of ～21 nucleotides in samples from a wide range of animal species, including vertebrate, ascidian, hemi-chordate, mollusc, annelid and arthropod, but not in RNAs from several cnidarian and poriferan species, Saccharomyces cerevisiae, Escherichia coli or Arabidopsis. We did not detect lin-4 RNA in these species. We found that let-7 temporal regulation is also conserved: let-7 RNA expression is first detected at late larval stages in C. elegans and Drosophila, at 48 hours after fertilization in zebrafish, and in adult stages of annelids and molluscs. The let-7 regulatory RNA may control late temporal transitions during development across animal phylogeny.
机译：两个小RNA调节秀丽隐杆线虫的发生时间。从第一阶段到第二阶段的幼虫命运的转变需要22个核苷酸的lin-4 RNA，而从后期的幼年阶段到成年细胞命运的转变则需要21个核苷酸的let-7 RNA。 lin-4和let-7 RNA基因彼此不是同源的，但它们分别与通常由RNA负调控的一组蛋白质编码靶基因的3'非翻译区中的序列互补。在这里，我们从多种动物物种（包括脊椎动物，海鞘，半柠檬酸盐，软体动物，无节肢动物和节肢动物）的样品中检测到约21个核苷酸的let-7 RNA，但未在几种虫和poriferan物种，酿酒酵母中的RNA中检测到。 ，大肠杆菌或拟南芥。我们没有在这些物种中检测到lin-4 RNA。我们发现，let-7的时间调控也得到了保留：let-7 RNA的表达首先在秀丽隐杆线虫和果蝇的幼虫后期，斑马鱼受精后的48小时以及成年幼体和软体动物中检测到。 let-7调节性RNA可能控制整个动物系统发育过程中的晚期时间过渡。

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