Modulation of cardiac ryanodine receptor 2 by calmodulin

Gong Deshun; Chi Ximin; Wei Jinhong; Zhou Gewei; Huang Gaoxingyu; Zhang Lin; Wang Ruiwu; Lei Jianlin; Chen S. R. Wayne; Yan Nieng

首页> 外文期刊>Nature >Modulation of cardiac ryanodine receptor 2 by calmodulin

【24h】

Modulation of cardiac ryanodine receptor 2 by calmodulin

机译：钙调蛋白的心脏ryanodine受体2的调节

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The high-conductance intracellular calcium (Ca2+) channel RyR2 is essential for the coupling of excitation and contraction in cardiac muscle. Among various modulators, calmodulin (CaM) regulates RyR2 in a Ca2+-dependent manner. Here we reveal the regulatory mechanism by which porcine RyR2 is modulated by human CaM through the structural determination of RyR2 under eight conditions. Apo-CaM and Ca2+-CaM bind to distinct but overlapping sites in an elongated cleft formed by the handle, helical and central domains. The shift in CaM-binding sites on RyR2 is controlled by Ca2+ binding to CaM, rather than to RyR2. Ca2+-CaM induces rotations and intradomain shifts of individual central domains, resulting in pore closure of the PCB95 and Ca2+-activated channel. By contrast, the pore of the ATP, caffeine and Ca2+-activated channel remains open in the presence of Ca2+-CaM, which suggests that Ca2+-CaM is one of the many competing modulators of RyR2 gating.

机译：高导率细胞内钙（CA2 +）通道Ryr2对于心肌激发和收缩的偶联至关重要。在各种调节剂中，钙调蛋白（CAM）以Ca2 +依赖性的方式调节Ryr2。在这里，我们揭示了通过人凸轮通过八条条件下的Ryr2的结构测定来调节猪Ryr2的调节机制。 apo-cam和ca2 + -cam与由手柄，螺旋和中心域形成的细长裂缝中的不同但重叠的位点。 Ryr2上的凸轮结合位点的偏移由Ca2 +与凸轮的结合控制，而不是Ryr2。 CA2 + -CAM诱导各个中央畴的旋转和纳内型偏移，导致PCB95和CA2 + -Activated通道的孔闭合。相比之下，在Ca2 + -CAM存在下，ATP，咖啡因和CA2 + -Activated通道的孔保持开放，这表明CA2 + -CAM是RYR2门控的许多竞争调制器之一。

著录项

来源
《Nature》 |2019年第7769期|347-351|共5页
作者
Gong Deshun; Chi Ximin; Wei Jinhong; Zhou Gewei; Huang Gaoxingyu; Zhang Lin; Wang Ruiwu; Lei Jianlin; Chen S. R. Wayne; Yan Nieng;
展开▼
作者单位

Tsinghua Univ Sch Life Sci Tsinghua Peking Joint Ctr Life Sci Beijing Adv Innovat Ctr Struct Biol Beijing Peoples R China;

Tsinghua Univ Sch Life Sci Tsinghua Peking Joint Ctr Life Sci Beijing Adv Innovat Ctr Struct Biol Beijing Peoples R China;

Univ Calgary Libin Cardiovasc Inst Alberta Dept Physiol & Pharmacol Calgary AB Canada;

Tsinghua Univ Sch Life Sci Tsinghua Peking Joint Ctr Life Sci Beijing Adv Innovat Ctr Struct Biol Beijing Peoples R China;

Tsinghua Univ Sch Life Sci Tsinghua Peking Joint Ctr Life Sci Beijing Adv Innovat Ctr Struct Biol Beijing Peoples R China;

Univ Calgary Libin Cardiovasc Inst Alberta Dept Physiol & Pharmacol Calgary AB Canada;

Univ Calgary Libin Cardiovasc Inst Alberta Dept Physiol & Pharmacol Calgary AB Canada;

Tsinghua Univ Sch Life Sci Technol Ctr Prot Sci Minist Educ Key Lab Prot Sci Beijing Peoples R China;

Univ Calgary Libin Cardiovasc Inst Alberta Dept Physiol & Pharmacol Calgary AB Canada;

Tsinghua Univ Sch Life Sci Tsinghua Peking Joint Ctr Life Sci Beijing Adv Innovat Ctr Struct Biol Beijing Peoples R China|Princeton Univ Dept Mol Biol Princeton NJ 08540 USA;

展开▼
收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类
关键词

相似文献

外文文献
中文文献
专利

1. Modulation of cardiac ryanodine receptor 2 by calmodulin [J] . Gong Deshun, Chi Ximin, Wei Jinhong, Nature . 2019,第7769期

机译：钙调蛋白对心脏ryanodine受体2的调节
2. Calmodulin binding sites of the skeletal, cardiac, and brain ryanodine receptor Ca2+ channels: modulation by the catalytic subunit of cAMP-dependent protein kinase? [J] . Guerrini R, Menegazzi P, Anacardio R, Biochemistry . 1995,第15期

机译：骨骼，心脏和大脑的雷诺丁受体Ca2 +通道的钙调蛋白结合位点：由cAMP依赖性蛋白激酶的催化亚基调节？
3. Modulation of the calmodulin-induced inhibition of sarcoplasmic reticulum calcium release channel (ryanodine receptor) by sulfhydryl oxidation in single channel current recordings and ((3)H)ryanodine binding. [J] . Suko J, Hellmann G, Drobny H The Journal of Membrane Biology: An International Journal for Studies on the Structure, Function & Genesis of Biomembranes . 2000,第2期

机译：钙调蛋白诱导的抑制肌质网钙释放通道（ryanodine受体）的巯基氧化在单通道电流记录和（（3）H）ryanodine绑定。
4. Ryanodine Receptors Coupling Causes a Calcium Leak in Cardiac Cell [C] . Alexander Ryvkin, Nikita Markov Computing in Cardiology Conference . 2018

机译：Ryanodine受体耦合在心脏细胞中引起钙泄漏
5. Structure Determination and Mechanistic Insights of: I.Cyanobacteriochrome NpR6012g4 Light Sensor Protein in Phototaxis II.Retinal Degeneration 3 (RD3) Protein in Vision III.Ryanodine Receptor 2 (RyR2) Regulation by Calmodulin (CaM) in Cardiac Function =结构测定和机理洞悉：I.趋光性中的蓝细菌色素NpR6012g4光敏蛋白 II.视觉作用中的视网膜退化蛋白3 III.心脏功能中的钙调蛋白调控兰诺定受体2 [D] . Yu, Qinhong. 2019

机译：Structure Determination and Mechanistic Insights of: I.Cyanobacteriochrome NpR6012g4 Light Sensor Protein in Phototaxis II.Retinal Degeneration 3 (RD3) Protein in Vision III.Ryanodine Receptor 2 (RyR2) Regulation by Calmodulin (CaM) in Cardiac Function =结构测定和机理洞悉：I.趋光性中的蓝细菌色素NpR6012g4光敏蛋白 II.视觉作用中的视网膜退化蛋白3 III.心脏功能中的钙调蛋白调控兰诺定受体2
6. The Arrhythmogenic Calmodulin p.Phe142Leu Mutation Impairs C-domain Ca2+ Binding but Not Calmodulin-dependent Inhibition of the Cardiac Ryanodine Receptor [O] . Mads Toft Søndergaard, Yingjie Liu, Kamilla Taunsig Larsen, 2017

机译：心律失常的钙调蛋白p.Phe142Leu突变损害C域Ca2 +绑定但不是钙调蛋白依赖性心脏Ryanodine受体的抑制作用。
7. Ca2+-dependent calmodulin binding to cardiac ryanodine receptor (RyR2) calmodulin-binding domains [O] . Malene Brohus, Mads T. Søndergaard, Sui Rong Wayne Chen, 2019

机译：Ca2 +依赖性钙调蛋白与心脏ryanodine受体（Ryr2）钙调蛋白结合结构域结合

Modulation of cardiac ryanodine receptor 2 by calmodulin

摘要

著录项

相似文献

相关主题

期刊订阅