• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nature >Structures of fungal and plant acetohydroxyacid synthases
【24h】

Structures of fungal and plant acetohydroxyacid synthases

机译：真菌和植物乙酰羟基酸合成酶的结构

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Acetohydroxyacid synthase (AHAS), also known as acetolactate synthase, is a flavin adenine dinucleotide-, thiamine diphosphate- and magnesium-dependent enzyme that catalyses the first step in the biosynthesis of branched-chain amino acids(1). It is the target for more than 50 commercial herbicides(2). AHAS requires both catalytic and regulatory subunits for maximal activity and functionality. Here we describe structures of the hexadecameric AHAS complexes ofSaccharomyces cerevisiaeand dodecameric AHAS complexes ofArabidopsis thaliana. We found that the regulatory subunits of these AHAS complexes form a core to which the catalytic subunit dimers are attached, adopting the shape of a Maltese cross. The structures show how the catalytic and regulatory subunits communicate with each other to provide a pathway for activation and for feedback inhibition by branched-chain amino acids. We also show that the AHAS complex ofMycobacterium tuberculosisadopts a similar structure, thus demonstrating that the overall AHAS architecture is conserved across kingdoms.Structures of the acetohydroxyacid synthase complexes ofSaccharomyces cerevisiaeandArabidopsis thalianaprovide insights into the biosynthesis of and feedback inhibition by branched-chain amino acids.
机译：乙酰羟基酸合酶（AHAs），也称为乙酰酸酯合酶，是黄素腺嘌呤二核苷酸 - ，硫胺素二磷酸和镁依赖性酶，其催化分支链氨基酸的生物合成中的第一步（1）。它是超过50种商业除草剂（2）的目标。 AHAS需要催化和调节亚基进行最大活动和功能。在这里，我们描述了酿酒酵母酿酒酵母的酿酒酵母酿酒酵母的葡萄球菌酿酒酵母的葡萄球菌的综合体的结构。我们发现这些AHAS复合物的调节亚基形成催化亚基二聚体所连接的核心，采用马耳他十字架的形状。该结构展示了催化和调节亚基如何彼此连通，以提供活化的途径和通过支链氨基酸进行反馈抑制。我们还表明，结核病的AHAS复合物具有类似的结构，因此展示了整体AHAS架构在界界界。乙酰羟基酸合酶络合物的结构酿酒酵母的结构甲基吡啶省综合症通过支链氨基酸的生物合成和反馈抑制的洞察力。

著录项

  • 来源
    《Nature》 |2020年第7828期|317-321|共5页
  • 作者

    Lonhienne Thierry; Low Yu Shang; Garcia Mario D.; Croll Tristan; Gao Yan; Wang Quan; Brillault Lou; Williams Craig M.; Fraser James A.; McGeary Ross P.; West Nicholas P.; Landsberg Michael J.; Rao Zihe; Schenk Gerhard; Guddat Luke W.;

  • 作者单位

    Univ Queensland Sch Chem & Mol Biosci Brisbane Qld Australia;

    Univ Queensland Sch Chem & Mol Biosci Brisbane Qld Australia;

    Univ Queensland Sch Chem & Mol Biosci Brisbane Qld Australia;

    Univ Cambridge Cambridge Inst Med Res Cambridge England;

    ShanghaiTech Univ Sch Life Sci & Technol Shanghai Inst Adv Immunochem Studies Shanghai Peoples R China;

    ShanghaiTech Univ Sch Life Sci & Technol Shanghai Inst Adv Immunochem Studies Shanghai Peoples R China;

    Univ Queensland Ctr Microscopy & Microanal Brisbane Qld Australia;

    Univ Queensland Sch Chem & Mol Biosci Brisbane Qld Australia;

    Univ Queensland Sch Chem & Mol Biosci Brisbane Qld Australia;

    Univ Queensland Sch Chem & Mol Biosci Brisbane Qld Australia;

    Univ Queensland Sch Chem & Mol Biosci Brisbane Qld Australia;

    Univ Queensland Sch Chem & Mol Biosci Brisbane Qld Australia;

    ShanghaiTech Univ Sch Life Sci & Technol Shanghai Inst Adv Immunochem Studies Shanghai Peoples R China|Nankai Univ Coll Life Sci State Key Lab Med Chem Biol Tianjin Peoples R China|Tsinghua Univ Lab Struct Biol Beijing Peoples R China;

    Univ Queensland Sch Chem & Mol Biosci Brisbane Qld Australia;

    Univ Queensland Sch Chem & Mol Biosci Brisbane Qld Australia;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号