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Coupling of activity, metabolism and behaviour across the Drosophila brain

机译：果蝇脑中活动，代谢和行为的偶联

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摘要

Coordinated activity across networks of neurons is a hallmark of both resting and active behavioural states in many species~(1-5). These global patterns alter energy metabolism over seconds to hours, which underpins the widespread use of oxygen consumption and glucose uptake as proxies of neural activity~(6,7). However, whether changes in neural activity are causally related to metabolic flux in intact circuits on the timescales associated with behaviour is unclear. Here we combine two-photon microscopy of the fly brain with sensors that enable the simultaneous measurement of neural activity and metabolic flux, across both resting and active behavioural states. We demonstrate that neural activity drives changes in metabolic flux, creating a tight coupling between these signals that can be measured across brain networks. Using local optogenetic perturbation, we demonstrate that even transient increases in neural activity result in rapid and persistent increases in cytosolic ATP, which suggests that neuronal metabolism predictively allocates resources to anticipate the energy demands of future activity. Finally, our studies reveal that the initiation of even minimal behavioural movements causes large-scale changes in the pattern of neural activity and energy metabolism, which reveals a widespread engagement of the brain. As the relationship between neural activity and energy metabolism is probably evolutionarily ancient and highly conserved, our studies provide a critical foundation for using metabolic proxies to capture changes in neural activity.
机译：跨神经元网络的协调活动是许多物种中休息和活跃的行为状态的标志〜（1-5）。这些全局模式改变了能量新陈代谢超过几秒钟，这使得氧气消耗和葡萄糖摄取的广泛使用作为神经活动的代理〜（6,7）。然而，神经活动的变化是否与与行为相关的时间尺度的完整电路中的代谢通量发生因果关系。在这里，我们将飞脑的两光子显微镜与传感器相结合，使得能够在休息和活跃的行为状态下同时测量神经活动和代谢通量。我们证明神经活动驱动代谢通量的变化，在这些信号之间产生紧密耦合，可以在脑网络上测量。使用局部视体扰动，我们证明了神经活性的瞬态增加导致细胞溶质ATP的快速和持续增加，这表明神经元代谢可预测分配资源，以期望未来活动的能源需求。最后，我们的研究表明，甚至最小的行为运动的启动导致神经活动和能量代谢模式的大规模变化，这揭示了大脑的广泛接合。随着神经活动和能量代谢之间的关系可能进化古老和高度保守，我们的研究为使用代谢代理捕获神经活动的变化提供了关键的基础。

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  • 来源
    《Nature》 |2021年第7858期|244-248|共5页
  • 作者

    Kevin Mann; Stephane Deny; Surya Ganguli; Thomas R Clandinin;

  • 作者单位

    Department of Neurobiology Stanford University;

    Department of Applied Physics Stanford University;

    Department of Neurobiology Stanford University|Department of Applied Physics Stanford University;

    Department of Neurobiology Stanford University;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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