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A proximity-dependent biotinylation map of a human cell

机译：人细胞的邻近依赖性生物素化图谱

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摘要

Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy(1) and biochemical fractionation coupled with mass spectrometry(2-4) have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of cellular compartments in living cells(5-7). Here we present a BioID-based map of a human cell on the basis of 192 subcellular markers, and define the intracellular locations of 4,145 unique proteins in HEK293 cells. Our localization predictions exceed the specificity of previous approaches, and enabled the discovery of proteins at the interface between the mitochondrial outer membrane and the endoplasmic reticulum that are crucial for mitochondrial homeostasis. On the basis of this dataset, we created humancellmap.org as a community resource that provides online tools for localization analysis of user BioID data, and demonstrate how this resource can be used to understand BioID results better.
机译：隔间化是真核细胞的定义特征，并将不同的生物化学过程分配到离散的亚细胞位置。显微镜（1）和与质谱（2-4）偶联的生物化学分级限定了各种不同细胞器的蛋白质片，但许多细胞内隔室对这些方法仍然是难治性的。邻近依赖性生物素化技术，例如Bioid提供了一种替代方法，以确定活细胞（5-7）中的细胞室组合物。在这里，我们在192个亚细胞标志物的基础上介绍了人类细胞的基于生物的地图，并在HEK293细胞中定义了4,145个独特蛋白质的细胞内位置。我们的本地化预测超过了先前方法的特异性，并使在线粒体外膜和内质网之间的界面处发现了蛋白质的发现，该内质网对线粒体稳态至关重要。在此数据集的基础上，我们将Humancellmap.org创建为社区资源，为用户生物数据的本地化分析提供在线资源，并展示该资源如何更好地理解BioID结果。

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  • 来源
    《Nature》 |2021年第7865期|120-124|共5页
  • 作者

    Go Christopher D.; Knight James D. R.; Rajasekharan Archita; Rathod Bhavisha; Hesketh Geoffrey G.; Abe Kento T.; Youn Ji-Young; Samavarchi-Tehrani Payman; Zhang Hui; Zhu Lucie Y.; Popiel Evelyn; Lambert Jean-Philippe; Coyaud Etienne; Cheung Sally W. T.; Rajendran Dushyandi; Wong Cassandra J.; Antonicka Hana; Pelletier Laurence; Palazzo Alexander F.; Shoubridge Eric A.; Raught Brian; Gingras Anne-Claude;

  • 作者单位

    Mt Sinai Hosp Lunenferd Tanenbaum Res Inst Sinai Hearth Syst Toronto ON Canada|Univ Toronto Dept Mol Genet Toronto ON Canada;

    Mt Sinai Hosp Lunenferd Tanenbaum Res Inst Sinai Hearth Syst Toronto ON Canada;

    McGill Univ Montreal Neurol Inst Montreal PQ Canada|McGill Univ Dept Human Genet Montreal PQ Canada;

    Mt Sinai Hosp Lunenferd Tanenbaum Res Inst Sinai Hearth Syst Toronto ON Canada;

    Mt Sinai Hosp Lunenferd Tanenbaum Res Inst Sinai Hearth Syst Toronto ON Canada;

    Mt Sinai Hosp Lunenferd Tanenbaum Res Inst Sinai Hearth Syst Toronto ON Canada|Univ Toronto Dept Mol Genet Toronto ON Canada;

    Mt Sinai Hosp Lunenferd Tanenbaum Res Inst Sinai Hearth Syst Toronto ON Canada|Univ Toronto Dept Mol Genet Toronto ON Canada|Hosp Sick Children Peter Gilgan Ctr Res & Learning Toronto ON Canada;

    Mt Sinai Hosp Lunenferd Tanenbaum Res Inst Sinai Hearth Syst Toronto ON Canada;

    Univ Toronto Dept Biochem Toronto ON Canada;

    Univ Toronto Dept Biochem Toronto ON Canada;

    Univ Toronto Dept Mol Genet Toronto ON Canada;

    Mt Sinai Hosp Lunenferd Tanenbaum Res Inst Sinai Hearth Syst Toronto ON Canada|Univ Laval Canc Res Ctr Big Data Res Ctr Dept Mol Med Quebec City PQ Canada|Univ Laval Res Ctr CHUL CHU Quebec Quebec City PQ Canada;

    Univ Hearth Network Princess Margaret Canc Ctr Toronto ON Canada|Univ Lille PRISM INSERM U1192 Villeneuve Dascq France;

    Mt Sinai Hosp Lunenferd Tanenbaum Res Inst Sinai Hearth Syst Toronto ON Canada;

    Mt Sinai Hosp Lunenferd Tanenbaum Res Inst Sinai Hearth Syst Toronto ON Canada;

    Mt Sinai Hosp Lunenferd Tanenbaum Res Inst Sinai Hearth Syst Toronto ON Canada;

    McGill Univ Montreal Neurol Inst Montreal PQ Canada|McGill Univ Dept Human Genet Montreal PQ Canada;

    Mt Sinai Hosp Lunenferd Tanenbaum Res Inst Sinai Hearth Syst Toronto ON Canada|Univ Toronto Dept Mol Genet Toronto ON Canada;

    Univ Toronto Dept Biochem Toronto ON Canada;

    McGill Univ Montreal Neurol Inst Montreal PQ Canada|McGill Univ Dept Human Genet Montreal PQ Canada;

    Univ Hearth Network Princess Margaret Canc Ctr Toronto ON Canada|Univ Toronto Dept Med Biophys Toronto ON Canada;

    Mt Sinai Hosp Lunenferd Tanenbaum Res Inst Sinai Hearth Syst Toronto ON Canada|Univ Toronto Dept Mol Genet Toronto ON Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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