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A transcriptomic atlas of mouse cerebellar cortex comprehensively defines cell types

机译：小鼠小脑皮质的转录组地图集全面地定义细胞类型

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摘要

The cerebellar cortex is a well-studied brain structure with diverse roles in motor learning, coordination, cognition and autonomic regulation. However,  a complete inventory of cerebellar cell types is currently lacking. Here, using recent advances in high-throughput transcriptional profiling~(1-3), we molecularly define cell types across individual lobules of the adult mouse cerebellum. Purkinje neurons showed considerable regional specialization, with the greatest diversity occurring in the posterior lobules. For several types of cerebellar interneuron, the molecular variation within each type was more continuous, rather than discrete. In particular, for the unipolar brush cells-an interneuron population previously subdivided into discrete populations-the continuous variation in gene expression was associated with a graded continuum of electrophysiological properties. Notably, we found that molecular layer interneurons were composed of two molecularly and functionally distinct types. Both types show a continuum of morphological variation through the thickness of the molecular layer, but electrophysiological recordings revealed marked differences between the two types in spontaneous firing, excitability and electrical coupling. Together, these findings provide a comprehensive cellular atlas of the cerebellar cortex, and outline a methodological and conceptual framework for the integration of molecular, morphological and physiological ontologies for defining brain cell types.
机译：小脑皮质是一种良好研究的大脑结构，具有多种在运动学习，协调，认知和自主调节中的角色。然而，目前缺乏完整的小脑细胞类型的库存。这里，利用最近的高通量转录分析〜（1-3）的进展，我们分子定义了成年小鼠细胞的单个小叶细胞类型。 Purkinje神经元显示出相当大的区域专业化，后叶片中最多的多样性。对于几种类型的小脑中型，每种类型内的分子变化更加连续，而不是离散的。特别地，对于单极刷细胞 - 先前细分为分立群体的中间核群 - 基因表达的连续变化与电生理学性能的分级连续相关。值得注意的是，我们发现分子层中间核由两种分子和功能性不同的类型组成。这两种类型都显示了通过分子层的厚度的形态变化的连续体，但电生理记录显示出自发烧制，兴奋性和电耦合的两种类型之间的显着差异。这些发现在一起提供了小脑皮层的综合性蜂窝成型，并概述了用于定义脑细胞类型的分子，形态和生理本体的方法和概念框架。

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  • 来源
    《Nature》 |2021年第7879期|214-219|共6页
  • 作者

    Velina Kozareva; Caroline Martin; Tomas Osorno; Stephanie Rudolph; Chong Guo; Charles Vanderburg; Naeem Nadaf; Aviv Regev; Wade G Regehr; Evan Macosko;

  • 作者单位

    Broad Institute of Harvard and MIT Stanley Center for Psychiatric Research;

    Broad Institute of Harvard and MIT Stanley Center for Psychiatric Research;

    Department of Neurobiology Harvard Medical School;

    Department of Neurobiology Harvard Medical School;

    Department of Neurobiology Harvard Medical School;

    Broad Institute of Harvard and MIT Stanley Center for Psychiatric Research;

    Broad Institute of Harvard and MIT Stanley Center for Psychiatric Research;

    Broad Institute of Harvard and MIT Stanley Center for Psychiatric Research;

    Department of Neurobiology Harvard Medical School;

    Broad Institute of Harvard and MIT Stanley Center for Psychiatric Research|Department of Psychiatry Massachusetts General Hospital;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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