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首页> 外文期刊>Nature >Electron currents generated by the human phagocyte NADPH oxidase.
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Electron currents generated by the human phagocyte NADPH oxidase.

机译：人吞噬细胞NADPH氧化酶产生的电子电流。

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摘要

Electron transport across biological membranes is a well-known feature of bacteria, mitochondria and chloroplasts, where it provides motive forces for vectorial transport processes. In contrast, electron transport is generally not found in the plasma membrane of eukaryotic cells, possibly because it would interfere with electric processes at the plasma membrane. An exception is provided by the phagocyte NADPH oxidase, which generates superoxide (O2.-) through electron transfer from cytosolic NADPH to extracellular oxygen. The enzyme is essential for host defence, and patients with chronic granulomatous disease, who lack the functional enzyme, suffer from severe infections. It has been suggested that electron transfer by the NADPH oxidase might be electrogenic. Here we demonstrate, using the whole-cell patch-clamp technique, the generation of electron currents by the NADPH oxidase in human eosinophil granulocytes. The currents were absent in granulocytes of sufferers of chronic granulomatous disease and under conditions of low oxygen. Generation of electron currents across the plasma membrane of eukaryotic cells has not been observed previously and might be-independently of the generation of superoxide-a physiologically relevant function of the phagocyte NADPH oxidase.
机译：电子跨生物膜的运输是细菌，线粒体和叶绿体的一个众所周知的特征，它为矢量运输过程提供了动力。相反，在真核细胞的质膜中通常找不到电子传输，可能是因为它会干扰质膜的电过程。吞噬细胞NADPH氧化酶是一个例外，它通过电子从胞质NADPH转移到细胞外氧而生成超氧化物（O2-。）。这种酶对于宿主防御至关重要，缺乏功能性酶的慢性肉芽肿病患者会受到严重感染。已经提出，NADPH氧化酶的电子转移可能是电致的。在这里，我们证明了使用全细胞膜片钳技术，在人嗜酸性粒细胞中由NADPH氧化酶产生电子电流。在低氧条件下，慢性肉芽肿病患者的粒细胞中没有电流。以前从未观察到跨真核细胞质膜的电子电流的产生，并且可能与超氧化物的产生无关，超氧化物的产生是吞噬细胞NADPH氧化酶的生理相关功能。

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