首页> 外文期刊>Nature >Increased NMDA current and spine density in mice lacking the NMDA receptor subunit NR3A.
【24h】

Increased NMDA current and spine density in mice lacking the NMDA receptor subunit NR3A.

机译:缺少NMDA受体亚基NR3A的小鼠的NMDA电流和脊柱密度增加。

获取原文
获取原文并翻译 | 示例
           

摘要

The NMDA (N-methyl-D-aspartate) subclass of glutamate receptor is essential for the synaptic plasticity thought to underlie learning and memory and for synaptic refinement during development. It is currently believed that the NMDA receptor (NMDAR) is a heteromultimeric channel comprising the ubiquitous NR1 subunit and at least one regionally localized NR2 subunit. Here we report the characterization of a regulatory NMDAR subunit, NR3A (formerly termed NMDAR-L or chi-1), which is expressed primarily during brain development. NR3A co-immunoprecipitates with receptor subunits NR1 and NR2 in cerebrocortical extracts. In single-channel recordings from Xenopus oocytes, addition of NR3A to NR1 and NR2 leads to the appearance of a smaller unitary conductance. Genetic knockout of NR3A in mice results in enhanced NMDA responses and increased dendritic spines in early postnatal cerebrocortical neurons. These data suggest that NR3A is involved in the development of synaptic elements by modulating NMDAR activity.
机译:谷氨酸受体的NMDA(N-甲基-D-天冬氨酸)亚类对于认为是学习和记忆基础的突触可塑性以及发育过程中突触的精炼至关重要。目前认为,NMDA受体(NMDAR)是异源多聚通道,其包含普遍存在的NR1亚基和至少一个局部定位的NR2亚基。在这里,我们报告主要在大脑发育过程中表达的调节NMDAR亚基NR3A(以前称为NMDAR-L或chi-1)的表征。 NR3A与大脑皮层提取物中的受体亚基NR1和NR2共免疫沉淀。在非洲爪蟾卵母细胞的单通道记录中,将NR3A添加到NR1和NR2中会导致出现较小的单位电导率。小鼠中NR3A的基因敲除会导致NMDA反应增强,并在出生后早期的大脑皮层神经元中增加树突棘。这些数据表明NR3A通过调节NMDAR活性参与突触元件的发育。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号