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首页> 外文期刊>Nature >Drosophila oocyte localization is mediated by differential cadherin-based adhesion (see comments)
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Drosophila oocyte localization is mediated by differential cadherin-based adhesion (see comments)

机译：果蝇卵母细胞定位是由基于钙黏着蛋白的不同粘附介导的（见评论）

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摘要

In a Drosophila follicle the oocyte always occupies a posterior position among a group of sixteen germline cells. Although the importance of this cell arrangement for the subsequent formation of the anterior-posterior axis of the embryo is well documented, the molecular mechanism responsible for the posterior localization of the oocyte was unknown. Here we show that the homophilic adhesion molecule DE-cadherin mediates oocyte positioning. During follicle biogenesis, DE-cadherin is expressed in germline (including oocyte) and surrounding follicle cells, with the highest concentration of DE-cadherin being found at the interface between oocyte and posterior follicle cells. Mosaic analysis shows that DE-cadherin is required in both germline and follicle cells for correct oocyte localization, indicating that germline-soma interactions may be involved in this process. By analysing the behaviour of the oocyte in follicles with a chimaeric follicular epithelium, we find that the position of the oocyte is determined by the position of DE-cadherin-expressing follicle cells, to which the oocyte attaches itself selectively. Among the DE-cadherin positive follicle cells, the oocyte preferentially contacts those cells that express higher levels of DE-cadherin. On the basis of these data, we propose that in wild-type follicles the oocyte competes successfully with its sister germline cells for contact to the posterior follicle cells, a sorting process driven by different concentrations of DE-cadherin. This is, to our knowledge, the first in vivo example of a cell-sorting process that depends on differential adhesion mediated by a cadherin.
机译：在果蝇的卵泡中，卵母细胞总是在一组十六种生殖细胞中处于后位。尽管这种细胞排列对于随后形成胚胎的前后轴的重要性已得到了充分证明，但是导致卵母细胞后定位的分子机制尚不清楚。在这里，我们显示出同源粘附分子DE-钙黏着蛋白介导卵母细胞定位。在卵泡生物发生期间，DE-钙粘蛋白在种系（包括卵母细胞）和周围卵泡细胞中表达，在卵母细胞和后卵泡细胞之间的界面处发现DE-钙粘蛋白的浓度最高。镶嵌分析表明，正确的卵母细胞定位在种系和卵泡细胞中都需要DE-钙黏着蛋白，表明该过程可能涉及种系-体间的相互作用。通过分析具有卵泡滤泡上皮的卵泡中卵母细胞的行为，我们发现卵母细胞的位置由表达DE-钙黏着蛋白的卵泡细胞的位置决定，卵母细胞选择性地附着于其上。在DE-钙粘蛋白阳性滤泡细胞中，卵母细胞优先接触那些表达较高水平的DE-钙粘蛋白的细胞。基于这些数据，我们建议在野生型卵泡中，卵母细胞与其姊妹种系细胞成功竞争与后卵泡细胞的接触，这是由不同浓度的DE-钙黏着蛋白驱动的分选过程。据我们所知，这是细胞分选过程的第一个体内实例，该过程取决于钙黏着蛋白介导的差异粘附。

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