Tom5 functionally links mitochondrial preprotein receptors to the general import pore

Dietmeier K; Honlinger A; Bomer U; Dekker PJ; Eckerskorn C; Lottspeich F; Kubrich M; Pfanner N

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Tom5 functionally links mitochondrial preprotein receptors to the general import pore

机译：Tom5在功能上将线粒体前蛋白受体与一般的进口孔相连

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Most mitochondrial proteins are synthesized as preproteins on cytosolic polysomes and are subsequently imported into the organelle. The mitochondrial outer membrane contains a multisubunit preprotein translocase (Tom) which has receptors on the cytosolic side and a general import pore (GIP) in the membrane. Tom20-Tom22 and Tom70-Tom37 function as import receptors with a preference for preproteins that have amino-terminal presequences or internal targeting information, respectively. Tom40 is an essential constituent of the GIP, whereas Tom6 and Tom7 modulate the assembly and dissociation of the Tom machinery. Here we report the identification of Tom5, a small subunit that has a crucial role importing preproteins destined for all four mitochondrial subcompartments. Tom5 has a single membrane anchor and a cytosolic segment with a negative net charge, and accepts preproteins from the receptors and mediates their insertion into the GIP. We conclude that Tom5 represents a functional link between surface receptors and GIP, and is part of an 'acid chain' that guides the stepwise transport of positively charged mitochondrial targeting sequences.

机译：大多数线粒体蛋白是作为胞质多核糖体上的前蛋白合成的，随后被导入细胞器。线粒体外膜包含一个多亚基的前蛋白转位酶（Tom），该蛋白在胞质侧具有受体，在膜中具有一个一般的导入孔（GIP）。 Tom20-Tom22和Tom70-Tom37充当输入受体，分别偏爱具有氨基末端前序或内部靶向信息的前蛋白。 Tom40是GIP的重要组成部分，而Tom6和Tom7则可调节Tom机械的组装和分离。在这里，我们报告Tom5的鉴定，Tom5是一个小亚基，在导入预定用于所有四个线粒体亚区室的前蛋白中具有关键作用。 Tom5具有单个膜锚和带有负净电荷的胞质段，并接受来自受体的前蛋白并介导其插入GIP。我们得出结论，Tom5代表表面受体和GIP之间的功能连接，并且是“酸链”的一部分，该酸链指导带正电荷的线粒体靶向序列的逐步运输。

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《Nature》 |1997年第6638期|p.195-200|共6页
作者
Dietmeier K; Honlinger A; Bomer U; Dekker PJ; Eckerskorn C; Lottspeich F; Kubrich M; Pfanner N;
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作者单位

Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Germany.;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词
Amino Acid Sequence; Biological Transport; Carrier Proteins genetics metabolism; Cloning; Molecular; Fungal Proteins genetics metabolism; Membrane Proteins genetics metabolism; Mitochondria metabolism; Mitochondrial Membrane Transport Proteins; Molecular Sequence Data; Porins metabolism; Protein Precursors metabolism; Receptors; Cytoplasmic and Nuclear metabolism; Recombinant Fusion Proteins genetics metabolism; Research Support; Non-U.S. Gov't; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins;

机译：氨基酸序列;生物转运;载体蛋白遗传代谢;克隆;分子;真菌蛋白遗传代谢;膜蛋白遗传代谢;线粒体代谢;线粒体膜转运蛋白;分子序列数据;孔蛋白代谢;蛋白质前体代谢;受体;细胞质和核代谢;重组融合蛋白遗传代谢;研究支持;非美国政府;啤酒酵母;啤酒酵母蛋白;

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专利

1. The preprotein translocase of the outer mitochondrial membrane: receptors and a general import pore [J] . C. Meisinger, J. Brix, K. Model, Cellular and molecular life sciences: CMLS . 1999,第9a10期

机译：线粒体外膜的前蛋白转位酶：受体和一般的导入孔
2. Protein Import Channel of the Outer Mitochondrial Membrane: a Highly Stable Tom40-Tom22 Core Structure Differentially Interacts with Preproteins, Small Tom Proteins, and Import Receptors [J] . Chris Meisinger, Michael T. Ryan, Kerstin Hill, Molecular and Cellular Biology . 2001,第7期

机译：外部线粒体膜的蛋白质导入通道：高度稳定的Tom40-Tom22核心结构与前蛋白，小汤姆蛋白和导入受体差异相互作用。
3. The mitochondrial receptor complex: the small subunit Mom8b/Isp6 supports association of receptors with the general insertion pore and transfer of preproteins. [J] . A Alconada, M Kübrich, M Moczko, Molecular and Cellular Biology . 1995,第11期

机译：线粒体受体复合物：Mom8b / Isp6小亚基支持受体与一般插入孔和前蛋白转移的结合。
4. The Effects of Alkyl Linker Length and Pore Size on the Bifunctional Catalytic Activity of Amine-Functionalized Silica Materials for C-C Bond Formation [C] . Eric G. Moschetta, Nicholas A. Brunelli, Virginia E. Collier AIChE Annual Meeting . 2014

机译：烷基接头长度和孔径对C-C键形成胺官能化二氧化硅材料双官能催化活性的影响
5. Studies of the mitochondrial import receptor Tom70, preprotein targeting and import. [D] . Fan, Anna. 2011

机译：线粒体导入受体Tom70，前蛋白靶向和导入的研究。
6. Protein Import Channel of the Outer Mitochondrial Membrane: a Highly Stable Tom40-Tom22 Core Structure Differentially Interacts with Preproteins Small Tom Proteins and Import Receptors [O] . Chris Meisinger, Michael T. Ryan, Kerstin Hill, 2001

机译：线粒体外膜的蛋白质导入通道：高度稳定的Tom40-Tom22核心结构与前蛋白小Tom蛋白和导入受体差异相互作用。
7. Tom5 functionally links mitochondrial preprotein receptors to the general import pore [O] . Klaus Dietmeier, Angelika Hönlinger, Ulf Bömer, 1997

机译：Tom5功能齐全地将线粒体预蛋白受体与一般进口孔联系起来

Tom5 functionally links mitochondrial preprotein receptors to the general import pore

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