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首页> 外文期刊>Nature >Dynamin self-assembles into rings suggesting a mechanism for coated vesicle budding.
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Dynamin self-assembles into rings suggesting a mechanism for coated vesicle budding.

机译：动力蛋白自组装成环，提示包膜囊泡出芽的机制。

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摘要

DYNAMIN, a 100K member of the GTPase superfamily, is the mammalian homologue of the Drosophila shibire gene product. Mutations in shibire cause a defect in endocytosis leading to accumulation of coated pits and deep invaginations at the plasma membrane of all tissues examined. Similarly, invaginated coated pits accumulate in mammalian cells overexpressing dominant-negative mutants of dynamin, establishing that dynamin is required for the formation of 'constricted' coated pits and for coated vesicle budding. Whether dynamin functions in the classic GTPase mode as a molecular switch to regulate events leading to coated vesicle budding or instead actively participates as a mechanochemical enzyme driving coated vesicle formation is unclear. Here we show that dynamin spontaneously self-assembles into rings and stacks of interconnected rings, comparable in dimension to the 'collars' observed at the necks of invaginated coated pits that accumulate at synaptic terminals in shibire flies. We propose that invaginated coated pits become constricted by the assembly of dynamin into rings around their necks. A concerted conformational change would then close the rings and pinch off the budding coated vesicles.
机译：DYNAMIN是GTPase超家族的100K成员，是果蝇Shibire基因产物的哺乳动物同源物。抑制物的突变引起内吞作用的缺陷，从而导致被检查的所有组织的质膜积聚和深陷于凹陷。同样，过度表达的dynamin显性-阴性突变体在哺乳动物细胞中积累了被侵染的包膜凹坑，从而确定了dynamin是形成“狭窄”包膜凹坑和包被囊泡出芽所必需的。尚不清楚动力蛋白是否以经典的GTPase模式作为分子开关来调节导致包被囊泡出芽的事件，还是作为驱动包被囊泡形成的机械化学酶主动参与。在这里，我们显示出动力蛋白自发地自组装成环和相互连接的环的堆栈，其尺寸与在实蝇果蝇的突触末端积累的带涂层的凹坑的颈部观察到的“领”相当。我们建议通过将动力蛋白组装成围绕其颈部的环来限制已侵入的涂层凹坑。然后，一致的构象变化将闭合环并夹住萌芽的包被囊泡。

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