• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nature >Tubular membrane invaginations coated by dynamin rings are induced by GTP-gamma S in nerve terminals.
【24h】

Tubular membrane invaginations coated by dynamin rings are induced by GTP-gamma S in nerve terminals.

机译：GTP-γS在神经末梢诱导了被动力环覆盖的管状膜内陷。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The mechanisms through which synaptic vesicle membranes are reinternalized after exocytosis remain a matter of debate. Because several vesicular transport steps require GTP hydrolysis, GTP-gamma S may help identify intermediates in synaptic vesicle recycling. In GTP-gamma S-treated nerve terminals, we observed tubular invaginations of the plasmalemma that were often, but not always, capped by a clathrin-coated bud. Strikingly, the walls of these tubules were decorated by transverse electron-dense rings that were morphologically similar to structures formed by dynamin around tubular templates. Dynamin is a GTPase implicated in synaptic vesicle endocytosis and here we show that the walls of these membranous tubules, but not their distal ends, were positive for dynamin immunoreactivity. These findings demonstrate that dynamin and clathrin act at different sites in the formation of endocytic vesicles. They strongly support a role for dynamin in the fission reaction and suggest that stabilization of the GTP-bound conformation of dynamin leads to tubule formation by progressive elongation of the vesicle stalk.
机译：胞吐作用后突触小泡膜重新内在化的机制仍存在争议。因为几个囊泡运输步骤需要GTP水解，所以GTP-γS可能有助于鉴定突触囊泡回收中的中间体。在GTP-γS处理的神经末梢中，我们观察到了浆膜的肾小管内陷，通常但不总是被包被网格蛋白的芽覆盖。引人注目的是，这些小管的壁被横向电子致密环修饰，该环在形态上类似于动力蛋白围绕管状模板形成的结构。动力蛋白是一种与突触小泡内吞有关的GTP酶，在这里我们显示这些膜小管的壁而不是其远端对动力蛋白免疫反应呈阳性。这些发现表明，动力蛋白和网格蛋白在胞吞小泡形成的不同部位起作用。他们强烈支持动力蛋白在裂变反应中的作用，并表明，与GTP结合的动力蛋白构型的稳定可通过逐渐延长囊泡茎杆导致小管形成。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号