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首页> 外文期刊>Nature >A two-step mechanism for 5' and 3' splice-site pairing.
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A two-step mechanism for 5' and 3' splice-site pairing.

机译：5'和3'剪接位点配对的两步机制。

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摘要

A fundamental question in the splicing of precursor messenger RNA is how the 5' and 3' splice sites are recognized and paired during the splicing reaction. It has been proposed that spliceosome assembly in metazoan pre-mRNAs can be initiated through interaction between the 3' splice site and specific sequence elements on the downstream exon (an exonic enhancer or a 5' splice site). Pairing of the intronic 5' and 3' splice sites occurs subsequently. We report here that 5' and 3' splice sites located on separate synthetic pre-mRNA substrates can be efficiently trans-spliced if the 3' trans-splicing substrate contains these downstream sequence elements. Moreover, selection of the trans 5' splice site can occur after the second pre-spliceosomal complex A has assembled on the 3' trans-splicing substrate. Thus our data demonstrate that 5' and 3' splice-site pairing in metazoans can occur in two distinct steps.
机译：前体信使RNA剪接中的一个基本问题是在剪接反应过程中如何识别和配对5'和3'剪接位点。已经提出，后生动物前mRNA中的剪接体组装可通过3'剪接位点与下游外显子（外显子增强子或5'剪接位点）上的特定序列元件之间的相互作用来启动。内含子5'和3'剪接位点的配对随后发生。我们在这里报告说，如果3'反式剪接底物包含这些下游序列元件，位于单独的合成pre-mRNA底物上的5'和3'剪接位点可以被有效地反剪。而且，反式5'剪接位点的选择可以在第二剪接前复合体A组装在3'反剪接底物上之后进行。因此，我们的数据表明，后生动物中的5'和3'剪接位点配对可以在两个不同的步骤中发生。

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