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A general mechanism for transcriptional synergy by eukaryotic activators.

机译：真核激活物转录协同作用的一般机制。

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摘要

One of the important regulatory concepts to emerge from studies of eukaryotic gene expression is that RNA polymerase II promoters and their upstream activators are composed of functional modules whose synergistic action regulates the transcriptional activity of a nearby gene. Biochemical analysis of synergy by ZEBRA, a non-acidic activator of the Epstein-Barr virus (EBV) lytic cycle, showed that the synergistic transcriptional effect of promoter sites and activation modules correlates with assembly of the TFIID:TFIIA (DA) complex in DNase I footprinting and gel shift assays. The activator-dependent DA complex differs from a basal DA complex by its ability to bind TFIIB stably in an interaction regulated by TATA-binding protein-associated factors (TAFs). TFIIB enhances the degree of synergism by increasing complex stability. Similar findings were made with the acidic activator GAL4-VP16. Our data suggest a unifying mechanism for gene activation and synergy by acidic and non-acidic activators, and indicate that synergy is manifested at the earliest stage of preinitiation complex assembly.
机译：真核基因表达研究中出现的重要调控概念之一是RNA聚合酶II启动子及其上游激活物由功能模块组成，其功能协同作用调节附近基因的转录活性。 EBBRA裂解周期的非酸性激活物ZEBRA对协同作用的生化分析表明，启动子位点和激活模块的协同转录作用与DNase中TFIID：TFIIA（DA）复合物的组装有关我的足迹和凝胶位移分析。活化剂依赖性DA复合物与基础DA复合物的区别在于，它在TATA结合蛋白相关因子（TAF）调节的相互作用中稳定结合TFIIB的能力。 TFIIB通过增加复杂的稳定性来增强协同作用的程度。用酸性活化剂GAL4-VP16也得到了类似的发现。我们的数据表明了酸性和非酸性激活剂对基因激活和协同作用的统一机制，并表明协同作用在预复合物组装的最早阶段就表现出来了。

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