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Effects of brassinolide on the survival, growth and drought resistance of Robinia pseudoacacia seedlings under water-stress

机译:油菜素内酯对水分胁迫下刺槐幼苗存活,生长和抗旱性的影响

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We conducted field and pot experiments to investigate the effects of brassinolide on 1-year-old Robinia pseudoacacia L. seedlings. In the field experiment, seedling roots were soaked in brassinolide solutions containing 0–0.4 mg/l pure brassinolide before planting. Survival and growth of the seedlings were determined 8 months later. The results showed that soaking roots in brassinolide prior to planting significantly increased the survival and growth of seedlings. The best results were in the 0.2 mg/l brassinolide treatment. In the pot experiment, roots were soaked in 0–0.4 mg/l brassinolide before planting followed by a foliar application of brassinolide when the seedlings leafed out. After the seedlings were established, the soil water content in the pots was regulated to simulate drought conditions and various physiological parameters were measured. The results showed that treatment with 0.2 mg/l brassinolide decreased the transpiration rate, stomatal conductance and malondialdehyde (MDA) content of seedlings growing under moderate or severe water stress compared to untreated seedlings. Leaf water content, predawn water potential, soluble sugar content, free proline content, and superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities were all greater in water-stressed seedlings in the 0.2 mg/l brassinolide treatment compared to the control. The results indicate that the application of brassinolide can ameliorate the effects of water stress and enhance drought resistance of Robinia seedlings. Treatment of seedlings with brassinolide may be a useful management tool for afforestation projects in arid and semiarid areas.
机译:我们进行了田间和盆栽试验,以研究油菜素内酯对1岁的刺槐(Robinia pseudoacacia L.)幼苗的影响。在田间试验中,在播种前将幼苗的根浸入含有0-0.4 mg / l纯油菜素内酯的油菜素内酯溶液中。 8个月后确定幼苗的存活和生长。结果表明,在种植前将根浸入油菜素内酯可显着提高幼苗的存活和生长。最好的结果是0.2 mg / l的油菜素内酯处理。在盆栽试验中,在播种前将根浸在0-0.4 mg / l的油菜素内酯中,然后在幼苗出叶时叶面喷施油菜素内酯。建立幼苗后,调节盆中的土壤水分以模拟干旱条件,并测量各种生理参数。结果表明,与未经处理的幼苗相比,以0.2 mg / l的油菜素内酯处理可降低中度或重度水分胁迫下幼苗的蒸腾速率,气孔导度和丙二醛(MDA)含量。在0.2 mg / l的油菜素内酯处理中,水分胁迫下的幼苗叶片水含量,黎明前的水势,可溶性糖含量,游离脯氨酸含量以及超氧化物歧化酶(SOD),过氧化物酶(POD)和过氧化氢酶(CAT)的活性均更高。到控制。结果表明,油菜素内酯的施用可以减轻水分胁迫的影响,增强刺槐幼苗的抗旱性。油菜素内酯处理幼苗可能是干旱和半干旱地区造林项目的有用管理工具。

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